摘要
目的 探讨细胞表面分化抗原CD117在各型白血病细胞上的表达规律及其意义。方法 采用CD4 5/SSC双参数散点图设门方法进行三色流式细胞术细胞表面分化抗原分析。结果 急性髓系白血病 (AML)患者CD117表达率为 6 8%,慢性粒细胞白血病急变期 (CML BC)患者CD117表达率为 80 %,而在急性淋巴细胞白血病中表达率极低 ,仅为 2 %。在慢性淋巴细胞白血病、CML慢性期中阴性。在AML中CD117主要表达在M0 /M1( 72 %)、M2 ( 88%)、M4 ( 5 0 %)、M5a( 75 %)、M6( 10 0 %) ,但在M3 和M5b中表达率较低 (分别为 39%和 2 9%) ,在M3 中CD117的表达率高于CD34 及HLA DR。CD117与CD14 在AML中的表达呈负相关。结论 CD117有助于淋巴系与髓系白血病的鉴别 ,并有助于白血病克隆与正常细胞的区分。
Objective To explore the expression of CD 117 on different types of leukemia and its significance. Methods CD 117 expression was analysed by three-color flow cytometry with CD 45/SSC gating strategy. Results CD 117 was expressed in 68% of acute myeloid leukemia (AML) and 80% of chronic myeloid leukemia in blast crisis (CML-BC) cases, but in only 2% of acute lymphoblastic leukemia cases. CD 117 was negative in CML in chronic phase and chronic lymphocytic leukemia. Cases in AML, CD 117 was expressed in 72% of M 0/M 1, 88% of M 2, 50% of M 4, 75% of M 5a, 100% of M 6 and 100% of M 7 cases but was less expressed in M 3 (39%) and M 5b (29%) cases. Expression of CD 117 was more frequent than CD 34 and HLA-DR in M 3 and some M 2 cases. There was a significant inverse relationship between CD 117 and CD 14 expression in AML. Conclusions Analysis of CD 117 expression may be of help in distinguishing myeloid from lymphoid leukemia and leukemia clone from normal cells.
出处
《中华血液学杂志》
CAS
CSCD
北大核心
2003年第5期228-230,共3页
Chinese Journal of Hematology
