摘要
目的 :探讨幽门螺杆菌L型(Hp_L,即球形Hp)感染与食管癌MVD、VEGF、P53表达的相关性及Hp_L对食管癌生长、浸润和转移等生物学行为的影响。 方法 :用革兰染色和免疫组化ABC法 ,检测98例食管癌和30例对照组的Hp_L;用免疫组化SP法检测上述组织的MVD值和VEGF、P53表达 ,分析Hp_L与MVD(微血管密度)、VEGF(血管内皮生长因子)和P53表达以及临床病理因素的关系。 结果 :癌组的Hp_L阳性率、MVD值以及VEGF、P53表达阳性率均高于正常组(P<0.005～P<0.001) ;癌组中Hp_L阳性组的MVD值、VEGF和P53表达阳性率也高于其Hp_L阴性组(P<0.005～P<0.001) ;Hp_L阳性与MVD呈正相关(r=0.46,P<0.01) ,与VEGF和P53表达呈正相关(r=0.31,P<0.01)。癌组的Hp_L阳性数与癌细胞的血管侵袭、浸润深度、食管旁淋巴结转移及远处淋巴结转移相关 ,而与肿瘤大小无关。 结论 :Hp_L与食管癌的新生血管形成密切相关 ,是影响食管癌生长、浸润。
Purpose: To determine the correlation between Helicob acter pylori L_form (Hp_L) infection in human esophageal carcino ma (EC) and microvessel density(MVD), vascular endothelial growth factor(VEGF)and P53 expression, and to study the effect of H p_L on the malignant biological behaviors of EC. Methods: Hp_L was examined in 98 patients with EC and 30 controls using Gram stain and immunohistochemical (ABC method). VEGF, P53 pr otein and MVD were examined immunohistochemically (SP method), a nd their relationship to clinicopathologic factors was analyzed. Results: The positive rates of Hp_L, MVD, VEGF and P53 in cancer group were significantly higher than that in the norma l group (P<0.005～P<0.001). The positive rates of MVD,VEGF and P53 in Hp_L positive group of EC were significantly higher than that in Hp_L negative group (P<0.005～ P<0.001 ). The positive rates of Hp_L were correlated with MVD (r=0. 46, P<0.01), and with the expression of VEGF and P53 (r=0.31 , P<0.01). The positive cases of Hp_L in EC group correlated with vessel invasion, the depth of invasion, esophageal side, and distant lymph node metastasis, but the relation to tumo r size was not significant. Conclusion: Hp_L infection in EC is closely related to tumor angiogenesis, and may be one of the important factor leading to the EC growth, invasion and metastasis.
出处
《癌变·畸变·突变》
CAS
CSCD
2003年第2期75-78,F003,共5页
Carcinogenesis,Teratogenesis & Mutagenesis
基金
安徽省教育厅科研基金资助项目(No.2000j1163)
关键词
食管肿瘤
幽门螺杆菌
血管内皮生长因子
carcinoma, esophageal
helicobacter pylori
vascular endothelial growth factor
