摘要
目的探讨大蒜多糖对实验性病毒性心肌炎小鼠心肌酶的影响。方法采用BALB/C小鼠腹腔注射柯萨奇病毒B3(CVB3)建立实验性小鼠病毒性心肌炎模型。小鼠随机分为正常对照组、模型组、大蒜多糖Ⅰ组、大蒜多糖Ⅱ组、大蒜多糖Ⅲ组和黄芪组。注射病毒 1小时后给药 ,连续 7天 ,于第 8天处死存活鼠取材。观察小鼠存活率及心肌病理变化 ,测定血清心肌酶及心肌组织中的病毒滴度。结果大蒜多糖治疗组与模型组相比小鼠存活率明显增高 ,心肌组织病理损伤减轻 ,血清心肌酶含量和心肌组织中病毒滴度明显降低。
Objective To investigate the effect of Garlic Polysaccharide on serum cardiac enzyme in experimental mice viral myocarditis model caused by Coxsackie virus B 3(CVB 3).Methods Viral myocarditis model was created by intraperitoneal inoculation with Coxsackie virus B 3 solution and mice were randomly devided into six groups:the normal control group,the model control group,the Garlic Polysaccharide groupⅠ(GP groupⅠ),the Garlic Polysaccharide groupⅡ(GP groupⅡ),the Garlic Polysaccharide groupⅢ(GP groupⅢ)and the Astragalus membranaceus group(AM group).The mice of GP group and AM group were treated by Garlic Polysaccharide and Astragalus membranaceus respectively 1h after CVB 3 infection.Blood and heart samples were obtained on the 8th day after CVB 3 infection.The survival rate and the myocardial histopathologic changes were recorded and observed.The serum cardiac enzyme and the virus title of heart were also measured.Results The survival rate in GP group were higher than that of the model control group.The myocardial damage,the serum cardiac enzyme and the virus title of heart in GP group reduced more significantly than that of the model control group.Conclusion Garlic Polysaccharide may have the therapeutic effect on experimental mice viral myocarditis caused by CVB 3.
出处
《咸宁医学院学报》
2003年第1期1-4,共4页
Journal of Xianning Medical College
基金
湖北省自然科学基金资助项目 ( 2 0 0 1ABB16 6 )
