摘要
以罗丹明 B为印迹分子 ,丙烯酰胺为功能单体 ,乙二醇二甲基丙烯酸酯为交联剂 ,制备了对罗丹明 B有较好选择性的印迹聚合物 .利用静态平衡结合法和 Scatchard分析法研究了此印迹聚合物的结合能力和选择性 ,结果表明 ,以丙烯酰胺为功能单体的印迹聚合物中形成了两类不同的结合位点 ,计算了离解常数分别为 1 .87×1 0 - 5和 4.2 4× 1 0 - 4mol/L.对比罗丹明 S和罗丹明 6G的结合特性 。
A molecularly imprinted polymer was prepared using acrylamide as monomer,rhodamine B as template molecule and a large amount of ethyleneglycol dimethacrylate as the cross- linking agent.The bulk polymer obtained was investigated in equilibrium binding experiments to evaluate the molecular recognition and binding characteristics of the rhodamine B molecularly imprinted polymer.Scatchard analysis showed thattwo sites were produced in the polymermatrix and theirdissociation constants were calculated to be1 .87× 1 0 -5mol/L and4.2 4× 1 0 -4mol/L respectively.The substrate selectivity of im- printed polymer and non- imprinted polymer was investigated.The results showed that the imprinted polymer exhibited much higher affinity for rhodamine B among the tested compounds.Finally,fluores- cence spectra indicted the interaction between the template and binding sites in the polymer.
出处
《兰州大学学报（自然科学版）》
CAS
CSCD
北大核心
2003年第2期57-60,共4页
Journal of Lanzhou University(Natural Sciences)
