摘要
目的 :探讨冻储时间对冷冻精子复苏率的影响 ,以期找到最佳复苏时间。 方法 :取 88份正常供精者标本进行精液常规分析 ,精液用程序降温仪通过三步降温法冻存后置 - 196℃液氮中冻储 ,88份冻存标本随机分成 5组 ,分别于冻存后的 1d(Ⅰ组 ,n =14 )、7d(Ⅱ组 ,n =2 4 )、30d(Ⅲ组 ,n =19)、180d(Ⅳ组 ,n =18)、30 0d(Ⅴ组 ,n =12 )取出 ,37℃水浴复苏 ,再次进行精液常规分析。计算冷冻复苏率。 结果 :5组间的复苏率差异均无显著性 (P均 >0 .0 5 ) ,冻储时间与复苏率之间无相关性 (r=- 0 .0 5 ,P >0 .0 5 )。 结论 :程序降温仪冻存精液标本置 - 196℃液氮后 2 4h即可复苏分析 。
Objectives: To study the effect of cryopreservative period on the cryosurvival of human spermatozoa and find out the optimal recovery time of cryopreservation. Methods: Eighty eight semen samples were collected from normal donors and divided randomly into 5 groups according to the period of cryopreservative storage (1 d, 7 d, 30 d, 180 d, 300 d) in liquid nitrogen after being frozen by the programized, three step freezing method. Fresh and frozen thawed semen were examined by the routine analysis of semen and then the sperm recovery rate were calculated. Results: There were no significant differences in sperm recovery rate between group Ⅰ and the others ( P >0.05). The period of cryopreservative storage in liquid nitrogen had no correlation with the cryosurvival of human spermatozoa ( r = 0.05 , P > 0.05 ). Conclusions: It was indicated that freezing thawing after 24 h would be helpful to the screening of semen donors in batches for donor insemination of human sperm bank.
出处
《中华男科学杂志》
CAS
CSCD
2003年第2期122-123,共2页
National Journal of Andrology
