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苹果试管苗不定根起源及其发育状况的研究 被引量:8

Study on the Origination and Development of Adventitious Roots in Plantlets of Tissue—cultured Apple
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摘要 利用光学和扫描电子显微镜观察结果表明,经琼脂生根培养基诱导产生的长富—2苹果 (Malus pumila Mill.) 试管苗的不定根起源于维管形成层。这种不定根发育不良,根表面缺乏根毛,内部解剖构造为典型的初生结构。为避免因不定根吸收、运输能力差而影响试管苗移栽成活,宜采用通透性良好的基质进行瓶内或瓶外生根,可诱导出优质不定根。 In order to find out the reason affecting the successful planting of tissue-cultured apple, the observations were made by using both optical and scanning electron microscopes on the origination, structure and development of adventitious roots in Ghangfu-2 apple plantlets (Malus pumila Mill.) . The results indicated that the adventitious roots originated from the vascular cambium protruded either from the stem directly or from the callus. They had already connected with their mother plants in vascular tissue before they outstretched from the plants. The adventitious roots induced in the agar medium were short of root hairs and only had a typical primary structure, which was the adverse factor affecting the successful transplanting. Satisfactory adventitious roots were obtained by using vermiculite medium and other aerated media in rooting process.
出处 《河北农业大学学报》 CAS CSCD 北大核心 1992年第4期46-50,共5页 Journal of Hebei Agricultural University
关键词 不定根 试管苗 根毛 苹果 发育 adventitious root tissue-cultured plantlets root hair apple (Malus pumila Mill. )
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参考文献4

  • 1戴洪义,沈德绪,林伯年.“杭青”梨试管苗移栽前后叶片与根的外部形态结构之扫描电镜观察[J]莱阳农学院学报,1987(02).
  • 2李作轩,贾稊.茎尖培养苗试管外生根的初步研究[J]吉林农业大学学报,1983(03).
  • 3王凯基,张丕方,倪德祥,包慈华.海岸红杉离体茎培养中植株形成的细胞组织学研究[J]复旦学报(自然科学版),1981(02).
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