摘要
为研究拟除虫菊酯抗性机制 ,通过对溴氰菊酯敏感、抗性和高抗性家蝇的离体酯酶活力测定、酶反应动力学及不连续非变性凝胶电泳 ,观察酯酶在拟除虫菊酯抗性中的作用。结果显示 ,用 α- NA做底物酯酶活力和Vm 在三种品系家蝇中没有差异 ,两个抗性品系之间 Km 和 Vm 也没有差异 ,但敏感品系的 Km 分别高于两抗性品系 ;聚丙烯酰胺凝胶电泳表明其同功酶谱差异很大 ,SS有 6条带 ,R、 RR各有 5条副业 ,且三者之间带的位置和带型均发生改变。结论 ,拟除虫菊酯抗性家蝇酯酶性质发生改变 ,可能参与对拟除虫菊酯的代谢抗性。
To investigate the role of esterase in pyrethroid resistnce of housefly, we have detected the activity, kinetic parameters and the polyacryl gel electrophoresis spectrum of the esterase from the susceptible, resistant and super resistant strains. The results show that the activity and the Vm of the esterase in the three strains are similar. The Km of the esterase from rseistant and super resistant strains is also similar. But the Km of the esterase in the susceptible strain is much higher. Further polyacryl gel electrophoresis reveals that the estrase from different strains involves different isoenzyme composition. Finally, the conclusion is drawn that the changes of esterase isoenzymes is involved in the mechanisms for pyrethroid resistance in housefly.
出处
《医学动物防制》
2003年第3期133-135,共3页
Journal of Medical Pest Control
