摘要
本文介绍超氧化物歧化酶(SOD)的氯甘脲和氯胺T标记方法的比较,结果表明:SOD用氯甘脲标记法^(131)I标记率(55.34％)较同样条件下氯胺T标记法^(131)I标记率(42.37％)为高;而且用氯甘脲标记方法的SOD酶的活性(0.5 U/μg)较氯胺T标记方法的SOD酶活性(0.2U/μg)要高。且前者具有操作简单、经济、高效、快速的优点。
A comparison of radioiodine labelling methods of SOD with iodogen and chloramine T is reported. The result indicated that the radioiodine labelling rate for the former (55.34%) was higher than that for the latter (42.37%) at the same condition, and the enzyme activity for the former (0.5U/μg) was also higher than that for the latter (0.2U/μg). And the former is a simple, economic, efficient and speedy method.
出处
《核技术》
CAS
CSCD
北大核心
1992年第2期125-128,共4页
Nuclear Techniques
关键词
氯甘脲
放射性
碘标记
氯胺T
SOD
Superoxide dismutase Iodogen Radioiodine labelling Chloramine T
