摘要
从转录水平上对原有载体进行改造,以期提高外源蛋白的表达量,为进一步研究改建载体后蛋白表达量奠定基础。将pBI-EGFP载体上的反应元件Pbi-1切下,连在pTet-On载体上PCMV的下游;然后将外源基因BMP-7重组到原有的pBI-EGFP载体内,进行酶切和序列鉴定。成功地构建了pTet-On-Pbi-1载体和真核表达载体pBI/BMP-7。
To reform the vector from the transcriptional level and to improve the protein expression,and make a base for further research on expression.The reaction element Pbi-1was cut from the vector pBI-EGFP,and then was put downstream the PCMV promoter of the vector pTet-On,the BMP-7gene was cloned into the vector pBI-EGFP and was appraised.The results were the successful construction of the vector pTet-On-Pbi-1and the eu-karyotic expression vector pBI /BMP-7.
出处
《生物技术通讯》
CAS
2003年第2期113-115,共3页
Letters in Biotechnology
基金
山东省科委重大攻关项目(981154506)
