摘要
随机引物指非特异序列的寡聚核苷酸作为ＤＮＡ合成过程中的引物，是相对于特异引物的概念９０年代它与ＰＣＲ技术结合衍生了几项新技术：采用不同长度随机引物进行ＤＮＡ指纹分析而衍生出的ＲＡＰＤ、ＡＰ－ＰＣＲ及ＤＡＦ方法；进行ｍＲＮＡ多态分析的“差异显示”；以及ｒＰＣＲ，Ｔ－ＰＣＲ等技术．以ＲＡＰＤ为例介绍了随机引物ＰＣＲ的技术特点及其在分子生物学研究中的应用．
Random or arbitrary primers; incontrast with common specific primer;refer tothe non-specific oligonucleotides used as primersin DNA synthesis.In 1990's several new techniques were developed as a result of combinationof random primers and PCR. RAPD; AP-PCRand DAF by random primers with differentlength were used for DNA fingerprinting.Differential display was used for polymorphismanalysis of mRNA.rPCR;T-PCR and so onderived from application of random primers werediscussed too.The technique characters andapplications in molecular biology research of random primered PCR were introduced particularlyin RAPD.
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
1996年第6期517-520,共4页
Progress In Biochemistry and Biophysics
关键词
分子生物学
随机引物
任意引物
random primer
arbitrary primer
PCR
molecular biology
