摘要
为了探讨三七皂苷 (PNS)对人骨髓CD34+ 造血干 /祖细胞的刺激增殖和诱导分化的作用 ,用DynalM 4 5 0CD34+ 免疫磁珠阳性选择法获取高纯度的人骨髓CD34+ 细胞 ,采用多向祖细胞 (CFU Mix)体外集落培养和流式细胞术检测细胞增殖与分化。结果显示 :经免疫磁珠阳性选择 ,从骨髓细胞收获的CD34+ 细胞获得率为 (1.0 3±0 .74 ) % ,流式细胞术分析纯度达到 86 % - 93%。PNS 10mg/L和 2 5mg/L能刺激CD34+ 细胞增殖 ,使CFU Mix集落生成增加 ,2 5mg/L的PNS对CFU Mix产率提高 (34.7± 16 .0 ) % (P <0 .0 1) ,是促进造血的最适浓度 ;PNS2 5 ,5 0和 10 0mg/L时 ,能诱导CD34+ 细胞向粒系细胞分化 ,粒系表面标记CD33+ 和CD15 + 的细胞百分比均明显高于无PNS的对照组 ,而红系细胞表面标记CD71+ 和G A+ 细胞百分比则无明显变化。结论 :PNS对CD34+ 造血干 /祖细胞不但具有显著的刺激增殖作用 。
The object of this study was to explore the effects of Panax notoginosides (PNS) on proliferation and differentiation of human CD34 + stem/progenitor cells. CD34 + cells were isolated from human bone marrow by using immune beads of Dynal M 450 system. The cells were exposed to PNS at different concentrations in both liquid and semi solid culture for 14 days. The cells were marked with monoclonal antibodies and analyzed by flow cytometry after culture. The CFU Mix colony formation from CD34 + cells was assayed. The results showed that: (1) The yield of CD34 + cells after being selected by immune beads were (1.03±0.74)% out of bone marrow nuclear cells with purity of 86%-93%. (2) PNS (10-25 mg/L) stimulated the proliferation of CD34 + cells, and raised the colony numbers of CFU Mix obviously in vitro. PNS 25 mg/L was the optimal concentration to promote proliferation of CD34 + cells, the increasing rate of CFU Mix colony was (34.7±16.0)%. (3) The differentiation of CD34 + cells was induced by exposure to PNS (25, 50 and 100 mg/L) in liquid culture for 14 days. The percentages of CD33 + and CD15 + cells were increased after PNS exposure, which were significantly higher than those of control(P<0.01), however CD71 + and G A + cells were no obviously difference after PNS treatmant. In conclusion, Panax notoginosides not only promote the proliferation of CD34 + cells, but also induce the differentiation committed to granulocytes.
出处
《中国实验血液学杂志》
CAS
CSCD
2003年第2期120-123,共4页
Journal of Experimental Hematology
基金
国家自然科学基金资助项目 编号 3 0 0 70 93 3
