摘要
目的 探讨聚合酶链反应 (PCR)技术临床应用于棘阿米巴角膜炎诊断的可行性及优越性。方法 以一段特异性通用引物并配合热启动聚合酶链反应技术来检测临床标本中的棘阿米巴原虫。结果 在门诊初诊为棘阿米巴角膜炎的 13例 (13只眼 )中 ,通过 PCR检测法有 11只眼证实为棘阿米巴原虫感染 ,同时角膜刮片染色后镜检有 5例发现了棘阿米巴包囊。
Objective To discuss the feasibility and superiority of diagnosis of acanthamoeba Keratitis using polymerase chain reaction.Methods We used a hot initiated PCR based method with asset of universal acanthamoeba specific primers to detect acanthamoeba.Result In13 eyes of 13 cases which had been diagnosed acanthamoeba in clinic 11 eyes proved acanthamoeba keratitis using PCR,5 eyes found acanthamoeba follicle by observing the epithelial specimen under microscope.Conclusion Polymerase chain reaction is of great importance in the detection of acanthamoeba keratitis.
出处
《临床眼科杂志》
2003年第2期124-125,共2页
Journal of Clinical Ophthalmology
