摘要
目的 :明确在人成牙本质细胞样细胞内c -Myc可否调控端粒酶活性。 方法 :将c -Myc基因转染至原代人成牙本质细胞样细胞样细胞 ,G418筛选后检测细胞内c -Myc和端粒酶逆转录酶 (telomerasereversetranscrip tase ,TERT)的表达以及端粒酶活性。结果 :c -Myc稳定整和入细胞并表达蛋白 ,该转染细胞在mRNA和蛋白质水平上TERT的表达上调 ,端粒酶活性转为阳性。结论 :在人成牙本质细胞样细胞样细胞内 ,外源性c -Myc基因可以通过上调TERT的表达水平而激活端粒酶 ,为建立永生化人成牙本质细胞样细胞系奠定了基础。
Objective:To understand whether the telomerase activity could be regulated by c-Myc in human odontoblast-like cells.Methods:Primary human odontoblast-like cells were cultured and transfected with c-Myc expressing vector.After selection with G418, integration and expression of c-Myc,expression of telomerase reverse transcriptase (TERT), and telomerase activity in transfected cells were detected.Results:After selection with G418 for four weeks, c-Myc was integrated and expressed stably at protein level.The telomerase activity and expression of TERT were upregulated in these transfected cells.Conclusions:Telomerase could be activated by exogenous c-Myc through upregulation of TERT expression in human odontoblast-like cells. This laid a foundation for establishing immortalized human odontoblast-like cell line.
出处
《口腔医学研究》
CAS
CSCD
2003年第2期88-91,共4页
Journal of Oral Science Research
基金
国家自然科学基金资助项目 (编号 :39970 792
30 2 70 374)
国家教育部回国人员启动基金资助项目 (编号 :2 0 0 0HG0 0 3)
