摘要
目的 :进一步探讨肾脏疾病患者血清中抗中性粒细胞细胞质抗体 (ANCA)的荧光模式及其意义。方法 :应用间接免疫荧光(IIF)和ELISA方法检测了 2 5 1例肾脏疾病患者血清中ANCA及其靶抗原。结果 :在 2 5 1例肾脏疾病患者中 ,ANCA阳性 5 1例占 2 0 .3% ,其中 10例为cANCA阳性 (10 / 2 5 1) ;30例为pANCA阳性 (30 / 2 5 1) ;11例为aANCA阳性 (11/ 2 5 1)。结论 :同时用IIF法和ELISA法检测ANCA ,可以提高ANCA检出的阳性率 ;不同ANCA荧光模式与疾病的种类病情及预后密切相关 ;提高对不典型ANCA和 pANCA伴ANA荧光模式的鉴别 。
AIM: To further explore significance of fluorescent patterns of antineutrophil cytoplasmic antibodies in renal diseases. METHODS: ANCA fluorescent patterns and its target antigens in sera of 251 patients with renal diseases were detected by indirect immunofluorescence (IIF) and ELISA. RESULTS: The positive percentage of ANCA is 20.3% (51/251), in which 3.98%(10/251) is cANCA, 11.95% (30/251) pANCA and 4.38%(11/251) aANCA. CONCLUSION: The simultaneous screening for ANCA and its target antigens by IIF and ELISA can increase the positive percentage of ANCA in renal diseases. This novel screening strategy is able to distinguish atypical ANCA(aANCA) from pANCA with ANA and therefore useful for differential diagnosis between vasculitis and autoimmune diseases.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2003年第2期185-186,189,共3页
Chinese Journal of Cellular and Molecular Immunology
