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聚丙烯酰胺梯度凝胶电泳胶板干燥保存法及幻灯片的制作 被引量:1

Methods for the Preserve of Dried Polyacrylamide Gradient Gel and to Make Slide
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摘要 聚丙烯酰胺梯度凝胶板经95%、100%乙醇脱水,然后可以经过两种方式处理:①自然干燥,再挂上一层火棉胶。②脱水后,流水冲至透明,再经二甲苯脱去一部分剩余水分,自然干燥。得到的干板可以长期保存。而透明后的干板又可制成幻灯片,供实验、教学等方面用。此法也可用于保存单一浓度的聚丙烯酰胺凝胶板。 A polyacrylamide gradient gel dehydrated by 95% and 100% ethanol can be treated with two methods: ① Following dehydration, gel was dried under natural condition and then covered with collodin. ② Dehydrated gel was washed with tap water till transparentness and thereafter redehydrated by xylene and dried under natural condition. Using above methods, gel can be preserved for a long time and the transparent one can be used to make slide which can be applied in research and teaching. These methods can be also used to preserve a polyacrylamide gel of single concentration.
出处 《哈尔滨医科大学学报》 CAS 1992年第2期151-152,共2页 Journal of Harbin Medical University
关键词 聚丙烯酰胺 凝胶板 纪灯片 Polyacrylamide gradient gel Preserve Slide made with dried gel
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  • 2余龙,宫立群,邓余,施少林,赵寿元.一种用于PCR-SSCP和STR分析的银染PAG胶膜片简易制备法[J].中华医学遗传学杂志,1996,13(3):177-178. 被引量:18
  • 3张晓楠 张延凤.凝胶干胶的实验台制作法[J].生物化学与生物物理进展,2000,27(1):104-104.
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  • 7Natalia N,Julieta M,Mareelo H,et al.Cost etticient potato(Solanum tuberosum L.)cultivar identification by microsatellite amplification[J].Potato Res,2002,45:131-138.
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