摘要
目的 建立能鉴别诊断恶性疟原虫间和日疟原虫的ＰＣＲ检测方法。方法 根据红内期疟原虫ＳＳＵｒＲＮＡ基因序列，设计合成两对引物，采用ＰＣＲ技术，检测８９份门诊“四热”病人血样，并与镜检法进行比较研究。结果 恶性疟原虫血样能扩增出２０５ｈｐ的特异带．间日疟原虫血样能扩增出１２０ｂｐ的特异带，ＰＣＲ检测的阳性率为７４．１６％，镜检法为６８．５４％，ＰＣＨ法与镜检法的符合率为９４．３８％。结论ＰＣＲ检测的敏感性和特异性均优于镜检法．
Aim To establish a PCR test to detect and differentiate Plasmodiu falciparum and Plasmodium vivax. Methods Two pair of oligonucleotide primers based on the sequence of the SSUrRNA gene was synthesized. With microscopic examination of blood smears as control, PCR was used to detect malaria in outpatient clinics (if China. Results The PCR product of P.falciparum cases was a 205bp fragment and the PCR product of P. vivax cases was a I2Ohp fragment. The positive rate of the PCR test(74. 16%) was significantly higher than that of microscopic examination(68. 54% ). The concordance rate between these two diagnostic methods was 94. 38%. Conclusions Ttie PCR test is specific, sensitive and has a great potential in identifying different Plasmodium species and detecting mixed infection.
出处
《热带医学杂志》
CAS
2001年第1期50-52,共3页
Journal of Tropical Medicine
基金
全军医药卫生科研基金资助(编号:96L034)
