摘要
目的 评价PCR检测技术在间日疟与恶性疟混合感染区诊断疟疾的现场应用价值。 方法 采集海南省疟疾混合感染流行区 3 0 4份滤纸干血滴样本 ,根据红内期疟原虫SSUrRNA基因序列 ,设计合成 3条引物 ,采用PCR技术在同一反应体系中扩增出间日疟原虫和恶性疟原虫不同的DNA片段 ,检测现场所采样本中的间日疟原虫或恶性疟原虫DNA ;同时与镜检法进行比较。 结果 在 3 0 4份样本中 ,PCR法阳性 15份 ,其中间日疟 7份 ,恶性疟 8份 ;镜检法阳性11份 ,其中间日疟 6份 ,恶性疟 5份。镜检阳性的样本PCR均为阳性 ;镜检阴性而PCR阳性的 4份样本 ,其扩增产物经限制性酶切鉴定 ,证实为间日疟原虫或恶性疟原虫DNA。 结论 此PCR检测体系灵敏、特异 ,对诊断或鉴别诊断间日疟原虫和恶性疟原虫混合感染具有实用价值。
Objective To evaluate the field application value of polymerase chain reaction(PCR) based method to detect and identify Plasmodium falciparum and Plasmodium vivax in mixed infection areas. Methods A total of 304 blood samples were collected in malaria mixed infection areas in Hainan Province. 3 primers were designed according to the plasmodium SSUrDNA sequence to detect the P. falciparum and P. vivax in a single tube by PCR and this PCR-based method was compared with the conventional microscopy method. Results Among 304 blood samples,15 samples were positive by PCR-based method(7 with P. vivax and 8 with P. falciparum ). However only 11 blood samples were positive (6 with P. vivax and 5 with P. falciparum ) by microscopy. The 4 blood samples,negative with microscopy but positive by PCR,were confirmed with plasmodium infection. Conclusion The PCR-based method was sensitive and specific. It’s useful in malaria field survey .
出处
《中国寄生虫病防治杂志》
CSCD
2004年第3期162-163,共2页
Chinese Journal of Parasitic Disease Control
关键词
聚合酶链反应
疟原虫
间日
疟原虫
恶性
混合感染
检测
Polymerase chain reaction
Plasmodium falciparum
Plasmodium vivax
mixed infection
detection
