摘要
自的 构建了三种表达形大的弓形虫主要表面抗原（ＳＡＧ１）基因－表膜型、分泌型和细胞内型，并分别用于免疫ＢＡＬＢ／Ｃ小鼠、结果ＳＡＧ１基因免疫小鼠后，表膜型和分泌型较细胞内型体液免疫出现早且反应强；细胞因子水平测试显示免疫后小鼠免疫反应趋向Ｔｈ１型。攻击感染结果证实：表膜型和分泌型免疫小鼠较细胞内型免疫小鼠所获保护力强。结论
Three forms of the major surface antigen (SAG1 )of Toxoplasma gondii. that is the membrane form, the secrete form and the intracellular form. were constructed and used to immunize BALB/c mice. The humoral response in the mice immunized with the membrane form and the secrete form of SAG1 appeared earlier and stronger than those mice immunized with the intracellular form. Result from the challenging infection demonstrated that the protection in the mice immunized with the membrane and the secrete forms was also stronger than in the mice immunized with the intracellular form. We suggest that the immune efficiency of the three forms of SAG1 in the mouse model is different.
出处
《热带医学杂志》
CAS
2001年第1期3-9,共7页
Journal of Tropical Medicine
