摘要
目的 探讨平滑肌肌动蛋白 (SMA) -α m RNA的表达 ,以了解其在哮喘发病机制中的作用。方法 以卵蛋白雾化复制哮喘模型 ,在激发后 3d、1周及 2周用逆转录聚合酶链反应 (RT- PCR)检测肺部α- SMA m RNA表达 ,同时用图象分析法测量大鼠气道形态学参数。结果 大鼠哮喘激发后 2周开始出现气道平滑肌肌层增厚 ,而α-SMA m RNA在哮喘激发 1周表达升高 ,2周后更加明显 ,α- SMA m RNA表达与气道平滑肌肌层增厚具有一定相关性。结论 哮喘气道重构中既有气道平滑肌的增生肥大 。
Objective To investigate the expression of smooth muscle actin(SMA) α mRNA in the lung of rat with asthma. Methods A rat model of asthma was established by exposing the animal to aerosolized ovalbumin (OA). After being sensitized, the rat was subjected to laboratory tests. α SMA mRNA of the lung tissue was measured by RT PCR. The thickness of the airway smooth muscle(ASM) was measured by an image analysis system at the same time. Results The thickness of ASM increased 2 weeks after OA challenge. The expression of α SMA mRNA increased 1 week after OA challenge and increased more significantly after 2 weeks. Conclusion In the rat model of asthma, hypertrophy and hyperplasia of the airway smooth muscle were noted, and the phenotype was changed.
出处
《四川大学学报（医学版）》
CAS
CSCD
北大核心
2003年第2期330-332,共3页
Journal of Sichuan University(Medical Sciences)
