摘要
目的 :观察重度妊高征 (SPIH)胎盘组织细胞凋亡现象 ,研究热休克蛋白 70 (HSP70 )及bax在胎盘组织细胞凋亡中的作用 .方法 :随机选取SPIH患者及正常晚孕胎盘组织各 2 5例 ,用光镜、电镜观察细胞凋亡现象 .免疫组化观察HSP70 ,bax在正常妊娠及SPIH胎盘组织中的表达 ,Westernblot检测HSP70在二者之间的蛋白表达差异 .结果 :在晚孕胎盘组织中可见到凋亡细胞 ,SPIH组细胞凋亡发生率的中位数及四分位数范围为 0 .3 6% (0 .2 0 % - 0 .4 6% ) ,正常对照组为 0 .15 %(0 .0 9% - 0 .2 1% ) .二者之间有显著性差异 (P <0 .0 5 ) .HSP70在SPIH组与对照组的阳性表达率分别为 10 0 %和 93 % ,无显著性差异 .但在表达强度上有明显的差别 (P <0 .0 1) .Westernblot的结果显示HSP70在SPIH胎盘组织中的表达增强 .bax在SPIH组的阳性率为 84 % ,对照组为 4 3 % ,有显著性差异 (P <0 .0 1) .结论 :SPIH胎盘组织细胞凋亡增多与bax的表达上调有一致性 ;HSP70不能抑制胎盘细胞凋亡 。
AIM: To study the relationship between the apoptosis and expression of heat shock protein 70 (HSP70) and bax in placentas of serious pregnancy induced hypertension (SPIH). METHODS: Placental samples were obtained from 25 uncomplicated third trimester pregnancies and from 25 cases of SPIH. Light microscopy and electron microscopy were used to identify and quantify apoptosis. HSP70 and bax were detected by immunohistochemical staining. The expression of HSP70 protein was assessed by Western blot. RESULTS: Apoptosis has been demonstrated within human third trimester placental tissues. Medians and interquartile ranges of SPIH group were 0.36% (0.20%-0.46% )and those of the normal control were 0.15% (0.09%-0.21%). The incidence of apoptosis was higher in placentas of SPIH (P<0.05). The positive rates of HSP70 were 100% in SPIH group and 93% in control, but the intensity of staining was significantly different between these two groups (P<0.01). In comparison to those in the normal control, the expression of HSP70 increased in placentas of SPIH group. The positive rates of bax were 84% in SPIH group and 43% in control, respectively ( P <0.01). CONCLUSION: Increased placental apoptosis in SPIH and the up regulation of expression of bax are simultaneous. The expression of HSP70 does not increase resistance to placental apoptosis in SPIH and it may play a role in the pathophysiologic mechanism of PIH.
出处
《第四军医大学学报》
北大核心
2003年第6期549-552,共4页
Journal of the Fourth Military Medical University
