摘要
选用抗寒性强弱不同的粳稻品种30份,进行胚及幼穗(0.5—1.0cm)体细胞组织培养。结果表明:来自不同品种的体细胞愈伤组织的净生长量,不仅在不同品种之间有明显差异,而且在继代培养中具有相对的稳定性;在15℃低温下继代培养的成熟胚愈伤组织净生长量与品种孕穗期的扰寒性无关,幼穗体细胞愈伤组织的净生长量与品种的抗寒性呈显著的正相关;将愈伤组织在15℃下培养25天,可作为愈伤组织抗寒性的鉴定方法,其净生长量可作为抗寒性鉴定的指标。
ABSTRACT The callcus cultures of somatic cell tissues from matur embryos and juvenile spikes (0.5~1.0 cm) of 30 rice varieties with different cold resistance were examined. The results showed that different genotypical callus had obvious difference in callus growth rate and Plantlet regenerative rate. And the differences relatively stabilized in continuous culturing. The Plantlet regenerative rates of calli from mature embryo were lower and not correlated with cold resistance of those varieties at 15℃.But plantlet regenerative rates of calli from jurenile spikes were higher and the positive correlation between callus growth rate and cold resistance of those rice varieties were significant. Thus, the callus growth rates of callus cultures developed at 15℃ for 25 days present as a morphological index to determine cold resistance of rice varieties and this might be suggested as a way for determining cold resistance of rice varieties.
出处
《吉林农业科学》
CSCD
北大核心
1989年第3期20-23,76,共5页
Journal of Jilin Agricultural Sciences
