摘要
目的 制备向革兰阳性细菌内有效转运药物的阳离子脂质体。方法 以荧光素钠为标志检测对象 ,以大豆磷脂、胆固醇、阳离子脂质为原料 ,依次进行旋转蒸发 -薄膜水化、超声分散、过膜挤压、冻干 ,制备荧光素钠阳离子脂质体。测定该脂质体的包封率和体外释药规律 ,流式细胞术 (FASC)检测该脂质体向金黄色葡萄球菌内转运的效率。结果 所得脂质体水合后粒径均匀 ,形态完整、规则 ,粒径范围 2 0～ 5 0nm ;3批脂质体的平均包封率为 19.4 2 %± 0 .31% ;体外释药呈现一级动力学规律 ,释药方程为Q =2 .82 93+2 .70 92T(r=0 .9839) ;FASC结果表明所制备的阳离子脂质体向细菌内转运率达 30 .1%± 12 .5 %。结论 制备的阳离子脂质体能够有效地向革兰阳性细菌内转运 。
OBJECTIVE To prepare uranine cationic liposome and evaluate the transfection efficiency on Staphylococcus Aureus. METHODS The liposomes were prepared by dehydration-rehydration, sonication, extrusion respectively and then lyophilized. The entrapment efficiency of uranine in the cationic liposome was detected and the transfection efficiency of the liposome on Staphylococcus Aureus was evaluated by FASC. RESULTS The results revealed that cationic liposome was regular in its morphology and the diameter range of the liposome was 20-50 nm. The mean entrapment efficiency of uranine in the liposome of three preparations was 19.42%. The release properties of the encapsulated uranine from liposome could be expressed by the following equation: Q=2.8293 + 2.7092T( r =0.9839). The results of FASC indicated that 30.1%±12.5% of Staphyloccus Aureus had integrated the encapsulated uranine.CONCLUSION The cationic liposome could be an idea vector for delivery of encapsulated drugs into Staphyloccus Aureus .
出处
《华西药学杂志》
CAS
CSCD
2003年第2期90-93,共4页
West China Journal of Pharmaceutical Sciences
基金
全军医药卫生科研基金 ( 0 2M0 0 8)
