摘要
目的 建立人脑胶质瘤裸鼠脑内原位移植动物模型并探讨其生物学特性。方法 采用瘤细胞悬液接种法 ,将人脑CHG 5胶质瘤细胞接种于裸鼠大脑实质内。分别于接种后 8、14、19、2 4和 3 0d处死动物并行病理检查、胶质纤维酸性蛋白免疫组化、染色体核型分析及透射电镜检查。结果 肿瘤移植成瘤率为 10 0 % ,肿瘤生长稳定 ,肿瘤病理学检查符合人脑胶质瘤细胞的形态学特征和免疫表型。结论 本移植瘤模型能作为研究胶质瘤发生、生物学特性以及治疗研究的可靠动物模型。接种后
Objective To establish an orthotopic implantation model of human glioma in nude mice and investigate its biological features. Methods The human CHG 5 glioma cells were inoculated into brains of nude mice. The animals were sacrificed at day 8, 14, 19, 24 and 30 after inoculation. The tumors were examined with light microscope, electron microscope, karyotype analysis and immunohistochemical stain for glial fibrillary acidic protein. Results Gliomas were formed in 100% in nude mice, and the growth of tumors was stable. The tumors showed the morphological features of human glioma with the immunophenotype. Conclusion The glioma model in nude mice is a reliable animal model for the study of the tumorigenesis and biological characteristics and therapy. The 14th day after inoculation might be suitable for experimental study.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2003年第4期287-290,共4页
Journal of Third Military Medical University
基金
国家自然科学基金资助项目 ( 39970 2 6 8)
