摘要
目的 观察不同正加速度 (+Gz)作用后大鼠血清中尿素氮 (BUN)、肌酐 (Cr)、碱性磷酸酶 (AKP)和Ca2 + 浓度及肾脏病理改变。方法 36只雄性Wistar大鼠随机分为 6组 ,每组 6只。A组为 +1Gz ,B组为 +5Gz ,C组和D组为 +7Gz,E组和F组为 +9Gz。B组、C组和E组在第 3次加速度作用 (每次 3min ,间隔 40min ,共 3次 )后6h处死。D组和F组在 2 4h后处死 ,测上述指标。结果 以A组为对照组 ,对照组的BUN、Ca2 + 和AKP分别为(9 2± 0 71)mmol L、(0 5 7± 0 2 9)mmol L和 (46 0± 74 0 6 )U L ,C组和E组的BUN[(10 18± 0 5 8)mmol L、(11± 0 35 )mmol L]有显著升高 (P <0 0 5 ,P <0 0 1) .B组、C组和E组的Ga2 + [(2 5± 0 5 4)mmol L、(3 1± 0 2 8)mmol L和 (3 0 7± 0 12 )mmol L]显著降低 (P <0 0 1、P <0 0 5和P <0 0 1)。E组的AKP(5 43 17± 2 2 7)U L有显著升高 (P <0 0 5 )。BUN是恢复至正常最快的肾功能指标。受 +7Gz和 9Gz重复作用后 ,C组和E组电镜下见肾小管上皮细胞 ,线粒体有病理变化。结论 正加速度对大鼠肾功能和超微结构有损害作用。
Objective To observe effect of different +Gz on renal function and its ultrastruture in rats.Methods Thirty-six male Wistar rats were randomly divided into six groups ( n =6 for each group).Group A served as ground control.Group B was exposed to +5 Gz while group C and group D exposed to +7 Gz, group E and group F exposed to +9Gz for 3 min in animal centrifuge (3 times,with 40 min interval in between).Blood samples of group B,C and group E were collected 6 h after +Gz exposure,and those of group D and group F collected 24 h after exposure,for the measurement of blood urea nitrogen (BUN), creatinine (Cr),alkaline phosphatase(AKP) and Ca 2+ levels. At the same time,the left kidney was taken for microscopic and electron microscopic examination.Results In comparision with group A, BUN in group C and group E were significantly elevated ( P <0.05). Ca 2+ levels in group B,C and group E were significantly decreased ( P <0.01, P <0.05 and P <0.01).AKP in group E was also significantly increased ( P <0.05).In electron microscopic examination, the pathological changes of mitochondria of glomeruli and renal tubules were found in rats after repeated +7 Gz and +9 Gz exposure.Conclusion Repeated +7 Gz and +9 Gz exposure may exert harmful effect on renal function and ultrastructure in rats.
出处
《中国比较医学杂志》
CAS
2003年第1期10-12,共3页
Chinese Journal of Comparative Medicine
