摘要
用强诱变剂 N-甲基-N-硝基-N-亚硝基胍（NG）处理条斑紫菜和坛紫菜的幼嫩叶状体，进行色素突变体诱导。结果表明：(1) 在相同实验条件下，两种紫菜的对照材料都未发现突变现象，而各诱变组都容易观察到突变的细胞或细胞块，表明在实验中发生的突变是诱变的结果。(2) 突变率随诱变剂量和诱变时间的增加而上升。在实验范围内，随着诱变强度的增加，条斑紫菜的突变率 从 最 低 的 11.2 % 上升到 28.7 %。坛紫菜从 10.1 % 上升到 20.2 %。（3）在同等面积的叶片上，诱变产生的突变型细胞数量，并不随诱变强度的增加而增加。实验使用定量视野检试，两种紫菜在各诱变组中出现的突变细胞数量基本相似，略高的突变值都发生在诱变实验的最低剂量组中。突变率表示突变细胞在存活细胞中所占的比例，而单位面积中的突变细胞数量，反映了诱变的效果。根据这一分析，可以认为 NG 浓度 25 μg/mL、诱变时间为 30 min 是合适的诱变条件。
：The young thalli of Porphyra yezoensis and P. haitanensis are induced by N-Methy-N-Nitro-N-Nitrosoguanidine (NG), a strong mutagen to induce the mutant of chromatophore. It is showed that no mutants have been investigated in all control groups, while in the induced groups, it is easy to find mutant cells or mutant cell masses in the thallus. The pigmentation mutants may be the result of NG inducement. The mutant rate increases obviously by increasing the reagent concentration or prolonging the induced time. Within the scope of experiment, the inducement effect of Porphyra yezoensis rises from 11.2 % to 28.7 %, and that of P. haitanensis from 10.1 % to 20.2 %. According to our experiments, the amount of mutant cells on the same area of thallus world not increase with inducement intensity. The amount of mutant cells in every group of inducement of both species of Porphyra changes very little with the fixed fields check method. The lowest dose (25μg/mL) of this experiment has the high mutant rate. The mutant rate shows the percentage of mutant cells in all the survived cells, while the amount of mutant cells reflect the effect of inducement. Thus the optimum inducement concentration of NG is 25μg/mL, and the optimum inducement time is 30 minutes.
出处
《海洋通报》
CAS
CSCD
北大核心
2003年第1期24-29,共6页
Marine Science Bulletin
基金
国家自然科学基金(C39770593)
中国科学院实验海洋生物学重点实验室资助
江苏省应用基础研究项目(BJ95182) 资助
关键词
紫菜
色素突变体
诱导
遗传分析
叶状体
亚硝基胍
： Porphyra
mutant of chromatophore
thallus
N-Methy-N-Nitro-N-Nitrosoguanidine(NG)
inducement
genetic analysis
