摘要
目的 探讨临床诊断为“非甲～戊型肝炎”患者中丙型肝炎病毒感染状况及基因型分析。方法 应用逆转录巢式 PCR(n PCR)法检测 HCV RNA,并对 HCV RNA核心区部分序列克隆测序分析。结果 6 0例临床诊断为“非甲～戊型肝炎”患者中 ,应用 n PCR法检测有 3例阳性 ,检出率为 5 %。3个 HCV北京分离株与 HCV 型核苷酸同源性为 94 %～ 96 %。基因进化树分析显示 3个北京分离株为 HCV 型。结论 临床诊断为“非甲～戊型肝炎”患者中 ,存在少数丙型肝炎病毒感染 ,其基因型以 HCV
Objective\ To genotype and detect HCV RNA in sera of patients with clinically diagnosed non A～E hepatitis.Methods\ HCV RNA was detected by reverse transcripted nested polymerase chain reaction (RT nPCR).Results\ Of 60 patients with clinically diagnosed non A～E hepatitis,3 were HCV RNA positive,the infected rate was 5%.Compared 3 HCV isolates from Beijing with reported HCV Ⅱ genotype,the nucleotides homologies were 94%～96%,3 HCV isolates were HCV Ⅱ genotype by phylogenetic tree.Conclusion The minority of patients with clinically diagnosed non A～E hepatitis are infected with hepatitis C virus,and its dominant genotype is HCV Ⅱ.
出处
《临床输血与检验》
CAS
2003年第1期15-18,共4页
Journal of Clinical Transfusion and Laboratory Medicine
基金
北京市委组织部优秀人才项目资助 (2 0 0 2 -0 4)
