摘要
目的 探讨一氧化氮合酶抑制剂、L 精氨酸对创伤性休克鼠血浆中的P 选择素的影响。方法 复制大鼠创伤性休克模型 ,于复苏时予以左旋 -N -位硝基精氨酸甲酯、氨基胍、L 精氨酸等治疗 ,采用酶联免疫法 (ELISA)测定大鼠动脉血浆中P 选择素的含量。结果 正常大鼠组动脉血浆中的P 选择素的含量较低 ;休克后 30min ,动脉血浆中的P 选择素含量较对照组升高 ,但无明显统计学意义 ;复苏后 6 0min ,动脉血浆中的P 选择素含量升高 ,与对照组比较有显著统计学意义 ;创伤休克 +L NAME组 ,复苏后 6 0min动脉血浆中的P 选择素含量较创伤休克 +盐水组上调 ;创伤休克 +AG组 ,复苏后 6 0min及复苏后 180min动脉血浆中的P 选择素变化不明显 ;创伤休克 +L Arg组 ,复苏后 6 0min动脉血浆中的P 选择素含量较创伤休克 +盐水组下调。结论 创伤性休克后体内P 选择素是升高的 ,这与休克时存在明显的微循环紊乱、内皮功能障碍、再灌流损伤和细菌移位有关 ;LNAME及L Arg对P 选择素有调节作用 ,而AG对P
Objective To investigate the changes of P selectin during traumatic shock by using nitric oxide synthase(NOS) and L Arginine(L Arg).Methods The rats were subjected to traumatic shock model.They were treated with N omega nitro L arginine methyl ester(LNAME),Aminoguanidin (AG)and L Arginine (L Arg)during resuscitation,and the arterial level of P selectin was determined with ELISA.Results The P selectin is low in plasma of normal rats.In traumatic shock group,30 min arter shock the P selectin level is insignificantly increased.60 min after resuscitation,the P selectin level is significantly increased.In LNAME group,the level of P selectin is significantly increased at 60 min after resuscitation.In L Arg group,the P selectin are significantly reduced at 60 min after resuscitation.In AG gruop there is no effect.Conclusion Arterial P selectin is increased in during traumatic shock.It is related to the marked microcirculatory perturbation and endothelial dysfunction and reperfusion injury and bacterial translocation during shock.The LNAME and L Arg can regulate the level of P selectin,but AG cannot. [
出处
《中华急诊医学杂志》
CAS
CSCD
2003年第2期100-102,共3页
Chinese Journal of Emergency Medicine
基金
广东省自然科学基金资助项目 0 0 10 48
