摘要
目的探讨创伤性休克时血浆及重要脏器P-选择素的分布、变化及其意义。方法复制大鼠创伤性休克模型,24只大鼠分为实验对照组、创伤休克组、左旋精氨酸(L-Arg 100 mg/kg·b.w.)治疗组,采用免疫组化方法(SABC法)检测大鼠心脏、肝脏、肺及小肠P-选择素的表达,同时采用ELISA测定大鼠血清中P-选择素水平。结果对照组大鼠重要脏器中P-选择素除了在肺中有少量表达外,其他脏器未见阳性表达,血清中的P-选择素有表达。休克后4 h,大部分脏器及血清中的P-选择素表达显著升高,L-Arg治疗组较休克组P-选择素明显下降,有统计学意义。结论创伤性休克可导致体内血清及重要脏器P-选择素大量表达,这与休克时存在明显的微循环紊乱和内皮功能障碍有关。L-Arg减少了P-选择素的合成其原因可能是促进了内皮性NO的合成。
Objective To investigate the changes of P-selectin distribution in the vital organs and plasma during traumatic shock and explore the significance of these changes. Methods Twenty-four normal SD rats were randomly assigned into 3 groups (n=8), namely traumatic shock group, L-arginine (L-Arg) treatment and control group. The rats in the former 2 groups were subjected to traumatic shock with L-Arg treatment group given 100 mg/kg L-Arg during resuscitation while the other receiving no medication. The control group received only intubation without trauma or phlebotomy. P-selectin expression in the vital organs including the heart, lungs, spleen, liver and small intestine was determined by means of streptavidin-biotin (SABC) immunocytochemical staining technique and serum P-selectin level assayed by enzyme-linked immunosorbent assay. Results Before traumatic shock, P-selectin was scarcely detected in the vital organs except the lungs, and the serum was positive for P-selectin expression. Four hours after shock, intense P-selectin expression was observed in almost all the vital organs and the serum P-selectin level was significantly higher than that of the control group. In L-Arg treatment group, P-selectin levels were significantly lowered than those of shock group. Conclusions P-selectin expression in the vital organs and serum is up-regulated during traumatic shock in rats, possibly due to severe microcirculatory disorder and endothelial dysfunction in this condition. L-Arg may decrease the expression of P-selectin very likely through promoting endothelial NO synthesis.
出处
《第一军医大学学报》
CSCD
北大核心
2003年第8期777-780,共4页
Journal of First Military Medical University
基金
广东省自然科学基金(001048)~~
