摘要
研究腺苷在利血平引起的大鼠行为性抑郁中的作用。应用Porsolt游泳试验 ,观察注射利血平引起的大鼠行为性抑郁 ,通过腹腔注射非特异性腺苷受体阻断剂咖啡因和特异性A1和A2腺苷受体阻断剂 ,确定腺苷在利血平诱导的大鼠行为性抑郁中的作用以及介导这种作用的受体。结果发现 :腹腔注射利血平 (4、6和 8mg/kg)可导致大鼠在游泳试验中漂浮时间明显延长 ,咖啡因和A2a腺苷受体阻断剂能明显缩短利血平导致的漂浮时间的延长。结论 :腺苷通过A2a受体介导利血平引起的大鼠的行为性抑郁。
Intraperitoneal injection of reserpine (4, 6 or 8 mg/kg) increased floating time in the Porsolt swim test in a dose and time dependent manner in rats. Although such behavioral depression usually is attributed to drug induced depletion of brain monoamines, the outcome might be more directly related to brain adenosine signaling associated neuronal overactivation or brain cytokine induction following excitotoxic tissue damage. We addressed these possibilities by pretreating rats with caffeine (7 mg/kg), a high affinity adenosine receptor antagonist, prior to reserpine treatment (6 mg/kg). Caffeine partially reversed the ensuing behavioral depression as measured in the Prosolt swim test conducted 1, 24 and 72 hours after reserpine treatment. Further investigation has also been done to determine the subtype of adenosine receptor, which should mediate reserpine's effect. The results showed that adenosine A2 receptor antagonist (DMPX) and A2a antagonist (CSC) reversed the reserpine induced behavioral depression dose dependently. These results suggest that adenosine mediates reserpine induced depression via adenosine A2a receptor and provide evidence that adenosine plays a crucial role in mediating behavioral depression, which will benefit in understanding the mechanism of depression and finding new drug for anti depressant treatment.
出处
《心理学报》
CSSCI
CSCD
北大核心
2003年第1期106-111,共6页
Acta Psychologica Sinica
