摘要
目的 探讨姜黄素对人肺癌细胞(SPC-A1)抗癌作用机制。方法 采用细胞培养、荧光显微镜、细胞凋亡检测、原位杂交等技术。结果 姜黄素处理后的癌细胞,光镜下可见有细胞脱壁,悬浮培养液中;荧光镜下可见细胞核破碎,裂解成大小不等的凋亡小体;20μmol/L姜黄素作用24 h凋亡率达43.67％,人肺癌细胞半胱氨酸蛋白酶8mRNA表达明显增高。结论 姜黄素可诱导人肺癌细胞凋亡,其作用机制可能与半胱氨酸蛋白酶8表达增高有关。
Objective To investigate the mechanism of anti-tumor effects of curcumin on human SPC-A1 cells.Methods By
using techniques of cell culture, fluorescence microscopy, in situ hybridization and cell apoptosis detection, the apoptosis of human SPC-A1 cells after treatment with curcumin were observed. Results After treatment with curcumin, SPC-A1 cells grew against the wall and then peeled off gradually. Fluorescent microscopy showed the nucleus broke into pieces, and cell split into apoptosis bodies in different size. The apoptotic cells reached 43.67% at the concentration of 20 fxmol/L curcumin at 24 h. The expression of caspase 8 mRNA in human SPC-A1 cells was up-regulated. Conclusion Curcumin can induce the apoptosis of SPC-A1 cells, and one of its molecular mechanisms may be associated with the up-regulation of caspase 8 expression.
出处
《华中医学杂志》
2003年第1期9-10,共2页
Central China Medical Journal
