摘要
应用 14个 10聚体随机引物对解放钟、森尾早生、早钟 6号、贵州野生、台湾枇杷、栎叶枇杷、乌脐、白梨、洛阳青、俨糖枇杷、湖北六二等 11个枇杷品种或种类的基因组DNA进行RAPD扩增 ,共得到 13 0条扩增带 ,其中 3 3条为非多态性条带 ,多态性程度为 74.62 %。进行聚类分析 ,以D1=0 .5 99进行划分时 ,可将枇杷的 11个遗传资源分成栽培和非栽培两个类群。以D2 =0 .64 9进行划分时 ,8个普通种可分为两个类群 ,即白肉类群和红肉类群 ,并在DNA分子水平上说明枇杷果肉色泽可作为分类的一个指标。
accessions of loquat genetic resources, including Eriobotrya japonica (cvs. Jiefangzhong, Moriowase, Zaozhong No.6, Wuqi, Baili, Luoyangqing, Yantangpipa, Hubeiliuer) E. prinoides, E. deflexa, Guizhouyesheng(wild loquat from Guizhou Province), were analyzed by RAPD with 14 arbitrary 10-mer primers. Total 130 DNA bands were amplified, among which 3 bands were common. The genetic diversity degree was 97.9%. By UPGMA (unweighted pair group with mathematic averages), given D1=0.599,11 loquat genetic germplasms were divided into 2 groups(cultivated group and un-cultivated group); given D2=0.649, E. japonica were divided into two groups (white-color and red-color pulp group). Moreover, the pulp color was proved to be a taxonomic index on DNA molecular level in loquat. RAPD technique has provided a new path for identification and classification of loquat genetic germplasm.
出处
《江西农业大学学报》
CAS
CSCD
2003年第2期258-261,共4页
Acta Agriculturae Universitatis Jiangxiensis
基金
福建省政府重点建设学科基金
福建省农科院果树所所长基金项目
