摘要
采用PCR RFLP技术检测了大约克、长白、杜洛克、宁乡、沙子岭和大围子 6个品种共 86 7头猪的E .coliF18受体 (ECF18R)基因座的遗传变异。结果表明 :Hin6Ⅰ RFLP位点上 ,大约克、长白、杜洛克 3个外来猪种均存在多态 ,且以敏感型 (GG型和AG型 )居多 ,平均占 94%,3个外来猪种的G等位基因频率平均为 0 76 ,AA抗性型个体占少数 ,平均为 6 %,猪群中M30 7处G→A的突变频率并不高。宁乡、沙子岭和大围子 3个本地猪种的所有检测样品都表现为GG型 ,在该位点上均不存在G→A的突变。各猪种ECF18受体基因座的PCR RFLP基因型分布 χ2 检验结果表明 ,每个外来猪种ECF18受体基因座的PCR RFLP基因型分布与 3个本地猪种的相比均差异显著或极显著 ,3个本地猪种间的ECF18受体基因座的PCR RFLP基因型分布完全一致。外来猪种间只有长白与杜洛克各基因型的分布差异显著 ,其余均不显著。
The genetic variation of E.coli F18 receptor(ECF18R)loci in 867 pigs including Large Yorkshire,Landrace,Duroc,Ningxiang pig,Shaziling pig and Daweizi pig was detected by PCR-RFLP with Hin6Ⅰ.The results showed as follows:The three exotic breeds including Large Yorkshire,Landrace and Duroc were proved to be polymorphic at the Hin6Ⅰ-RFLP site.The average susceptibility genotype frequency of AG and GG in the exotic breeds was 0.94,G allelic frequency was 0.76.The G→A mutation frequency at M307 site was very low in exotic breeds.The resistance genotype frequency of AA was 0.06.But the three native breeds including Ningxiang pig,Shaziling pig and Daweizi pig presented only GG genotype and no variation at the Hin6Ⅰ-RFLP site.The χ 2 value of ECF18R genotype distribution at the Hin6I-RFLP site in different pigs indicated that ECF18R genotype distribution of every exotic pig breed had significant or highly significant differences with three native pig breeds'.The ECF18R genotype distribution of three native pig breeds was the same completely.The others had no difference except that Landrace ECF18R genotype distribution had significant differences with Duroc's in the exotic breeds of pig.
基金
国家自然科学基金项目资助 (编号 :30 0 70 5 5 3)~~
