期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

猪α1岩藻糖转移酶基因点突变的PCR-SSCP检测 被引量：2

Detection of point mutation of porcine α1-fucosyltransferase gene by PCR-SSCP approach
在线阅读 下载PDF
导出
摘要 猪α1岩藻糖转移酶基因(FUT 1)位于猪第6号染色体上,是猪肠毒素大肠杆菌F 18受体(ETEC F 18R)理想的候选基因。采用PCR-SSCP法共检测外来猪种、国内地方猪种和培育猪种3个类型9个猪种共计255个个体FUT 1基因M 857位点的多态性,所检测的3个外来猪种均存在GG和AG两种基因型,而国内地方猪种中除临高猪外均表现为极端的单态分布,只存在GG基因型。采用PCR-SSCP方法对FUT 1基因点突变进行检测,具有试验成本低、操作简便和灵敏度高等优点,可望在育种实践中得到更广泛的应用。 αl-fucosyltransferase （FUT1） gene has been identified as a candidate gene for Enterotoxigenic Escherichia coli F18 receptor （ETEC F18R） and was located on the pig chromosome 6. By the PCR-SSCP method, the loci of 307 bp of FUT1 gene in 9 pig breeds for polymorphism were screened respectively. The results showed that the GG and AG genotypes were detected in three foreign breeds, however, the AG genotype could be detected only in Lingao pigs of all tested Chinese native pig breeds, and the others carried only genotype of M857^GG, and showed extremely abbreviation distribution. The method of PCR-SSCP was used in this research to detect the point mutation of FUT1 gene, this method has the advantage of low cost, easy manipulation, and high sensitivity. This method may be applied for commercial detection in swine breeding.
作者 吴圣龙 鞠慧萍 包文斌 陈国宏 朱国强 黄雪根 华金弟 李碧春 原志伟
机构地区 扬州大学动物科学与技术学院 扬州大学兽医学院 江苏省苏州市苏太猪育种中心
出处 《扬州大学学报（农业与生命科学版）》 CAS CSCD 北大核心 2006年第2期68-71,共4页 Journal of Yangzhou University：Agricultural and Life Science Edition
基金 江苏省高技术研究(农业)项目(BG2006302) 扬州大学自然科学基金资助项目(NK0513119)
关键词 猪 α1-岩藻糖转移酶基因 点突变 PCR—SSCP pig αl-fucosyltransferase gene point mutation PCR-SSCP
分类号 S852.612 [农业科学—基础兽医学] S858.28 [农业科学—临床兽医学]
  • 相关文献

参考文献10

  • 1Imberechts H,De Greve H,Schlicker C,et al.Characterization of F107 fimbriae of Escherichia coli 107/86,which causes oedema disease in pigs and nucleotide sequence of F107 major fimbrial subunit gene,fedA[J].Infect Immun,1992:60(5):1963-1971.
  • 2Bertschinger H U,Stamm M,Vogeli P.Inheritance of resistance to oedema disease in the pig:Experiments with an Escherichia coli strain expressing fimbriae 107[J].Vet Microbiol,1993,35(1-2):79-89.
  • 3Imberechts H,Bertschinger H U,Stamm M,et al.Prevalence of F107 fimbriae on Escherichia coli isolated from pigs with oedema disease or postweaning diarrhea[J].Vet Microbiol,1994,40(3-4):219-230.
  • 4Vogeli P,Meijerink E,Fries R,et al.A molecular test for the detection of E.coli F18 receptors:A breakthrough in the struggle against oedema and postweaning diarrhoea in swine[J].Schweiz Arch Tierheilk,1997,139(11):479-484.
  • 5Meijerink E,Fries R,Vogeli P,et al.Two α(1,2) fucosyltransferase genes on porcine chromosome 6q11 are closely linked to the blood group inhibitor(S) and Escherichia coli F18 receptor (ECF18R) loci[J].Mammalian Genome,1997,8:736-741.
  • 6施启顺,谢新民,柳小春,黄生强,贺长青.猪肠毒素大肠杆菌(ETEC)F18受体基因型检测报告[J].遗传,2002,24(6):656-658. 被引量：16
  • 7施启顺,黄生强,柳小春,贺长青,蒋隽.不同猪种E .coli F1 8受体基因的多态性[J].Acta Genetica Sinica,2003,30(3):221-224. 被引量：39
  • 8晏学明,任军,郭源梅,丁能水,陈克飞,高军,艾华水,陈从英,麻骏武,黄路生.猪a1-岩藻糖转移酶基因(FUT1)在26个中外猪种中的遗传变异研究(英文)[J].Acta Genetica Sinica,2003,30(9):830-834. 被引量：34
  • 9晏学明,郭源梅,丁能水,任军,黄路生.不同品种猪α1-岩藻糖转移酶基因遗传变异初析[J].中国畜牧杂志,2004,40(3):8-10. 被引量：18
  • 10王建业,孙怀昌,朱国强.禽腺联病毒的分离及其基因组鉴定[J].扬州大学学报（农业与生命科学版）,2005,26(2):7-10. 被引量：6

二级参考文献23

  • 1Sambrook J Fritsch EF Maniatis T.分子克隆实验指南（第2版）[M].北京:科学出版社,1998..
  • 2施启顺 谢新民 柳小春 等.中国动物遗传育种研究(第十一次全国动物遗传学术会议论文集)[C].北京:中国农业科技出版社,2001..
  • 3[2]Meijerink E,Fries R,Vogeli P,Masabanda J,Wigger G,Stricker C,Neuenschwander S,Bertschinger H U,Stranzinger G.Two α(1,2) fucosyltransferase genes on porcine chromosome 6q11 are closely linked to the blood group inhibitor(S) and Escherichia coli F18 receptor (ECF18R) Loci.Mammalian Genome,1997,8:736～741.
  • 4[3]Vogeli P,Bertschinger H U,Stamm M,Stricker C,Hagger C,Fries R,Rapacz J,Stranzinger G.Evidence for linkage of genes of the halothane(HAL) linkage group to the genes specifying receptors for F18 fimbriated Escherichia coli strains causing oedema disease and postweaning diarrhoea in Swiss Landrace pigs.Animal Genetics,1996,27:321～328.
  • 5[4]Edfors-Lilja,Wallgren P.Escherichia coli and Samonnella diarrhoea in pigs.Axford P F E,Bishop S C,Nicholas F W,Owen J O eds:Breeding for Disease Resistance in Farm Animals.CAB INTERNATIONAL,2000,253～267.
  • 6[6]Vogeli P,Meijerink E,Fries R,Stricker C,Bertschinger H U.A molecular test for the detection of E.coli F18 receptors:a breakthrough in the struggle against oedema and postweaning diarrhoea in swine.Schweiz Arch Tierheilk,1997,139(11):479～484.
  • 7Imberechts H, de Greve H, Schlicker C, Bouchet H, Pohl P, Charlier G,Bertschinger H, Wild P, Vandeke rckhove J, van Damme J. Characterization of F107 fimbriae of Escherichia coil 107/86, which causes oedema disease in pigs and nucleotide sequence of the F107 major fimbrial subunit gene, fedA. Infect Immun, 1992, 60 ( 5 ) : 1963-1971.
  • 8Vogeli P, Bertschinger H U, Stamm M, Stricker C, Hagger C, Fries R,Rapacz J, Stranzinger G. Genes specifying receptors for F18 fimbfiated Escherichia coli, causing oedema disease and postweaning diarrhoea in pigs,map to Chromosome 6. Anita Genet, 1996,27(5) :321 - 328.
  • 9Edwin Meijefink, Stefan Neuenschwander, Ruedi Fries, Andre.as Dinter, Hans U, Bertschinger Gerald Stranzinger, Peter Vogeli. A DNA polymorphism influencing a(1,2) fucosyltransferase activity of the pig FUT1 enzyme determines susceptibility of small intestinal epithelium to Escherichla coli F18 adhesion.Immunogenetics, 2000, 52 (1-2) :129- 136.
  • 10Klukowska J, Urbaniak B, Switonski M. High frequency of M307 (A)mutation at FUT1 locus, causing resistance to oedema disease, in an autochtonous polish pig breed, the Zlotnicka Spotted. Journal of Animal Breeding and Genetics, 1999,116(6):519- 524.

共引文献56

同被引文献22

  • 1韩正康,林玲,倪桂芝,陈杰.鹅生长过程中血清生长抑素水平变化的研究[J].畜牧兽医学报,1993,24(2):120-124. 被引量：4
  • 2刘春玲,丁利军,丁家桐.姜曲海母猪初情期前生殖器官雌激素受体mRNA表达丰度的研究[J].扬州大学学报（农业与生命科学版）,2005,26(1):33-36. 被引量：2
  • 3朱荣生,张牧,经荣斌,王宵燕,张金存,杨元青,陈华才.姜曲海瘦肉型品系母猪早期生殖器官发育及生殖激素分泌的研究[J].扬州大学学报（农业与生命科学版）,2005,26(3):35-39. 被引量：4
  • 4Kelm S,Schauer R.Sialic acids in molecular and cellular interactions,Int Rev Cytol,1997,175:137-240.
  • 5Benin AM,Ducher-Suchaux MF.Relationship between virulence and adherence of various enterotoxigenic Escherichia coli:strains to isolated intestinal epithelial cells from Chinese Meishan and European large white pigs.Am J Vet Res,1991,52(1):45-49.
  • 6Meijerink E,Fries R,Vogeli P,Masabanda J,Wigger G,Stricker C,Neuenschwander S,Bertschinger H U,Stranzinger G.Two a(1,2)fucosyitransferase genes on porcine chromosome 6q11 are closely linked to the blood group inhibitor(S)and Escherichia coli F18 receptor(ECF18R)Loci.Mamm Genome,1997,8(10):736-741.
  • 7Vogeli P,Meijerink E,Fries R,Stricker C,Bertschinger HU.A molecular test for the detection of E.coli F18 receptors:a breakthrough in the struggle against oedema disease and postweaning diarrhoea in Swine.Schweiz Arch Tierheilkd,1997,139(11):479-484.
  • 8Bao WB,Wu SL,Musa HH,Zhu GQ,Chen GH.Genetic variation at the alpha-1-fucosyltransferase(FUTI)gene in Asian wild boar and Chinese and Western commercial pig breeds.J Anim Breed Genet,2008,125(6):427-430.
  • 9Bertschinger HU,Pohlenz J.Bacterial colonization and morphology of the intestine in porcine Escherichia coli enterotoxemia(edema disease).Vet Pathol,1983,20(1):99-110.
  • 10Mouricout M,Vddrine B,Grange PH.Approach to the molecular basis and genetics of susceptibility resistance to colibacillosis in porcine species.In:Proc Inter Conf Anim Biol.1997,47-50.

引证文献2

二级引证文献6

扬州大学学报（农业与生命科学版）
2006年 第2期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部