摘要
目的 观察碱性成纤维细胞生长因子 (b FGF)能否通过胎盘屏障并干预宫内窘迫胎鼠脑损伤。 方法 15 d Wistar孕鼠 5只 ,经腹腔注射 1 2 5I标记的 b FGF(1 2 5I- b FGF) ,30 m in后取孕鼠血清、胎鼠脑、心、肝、脾、肺 ,检测各组织 1 2 5I- b FGF的放射活性 ;另取 32只怀孕 15 d Wistar孕鼠随机分为正常对照、窘迫对照、窘迫后干预及预防共四组 ,b FGF干预 ,免疫荧光双标记法标记增殖的神经元细胞。 结果 孕鼠注射 1 2 5I- b FGF 15 0 ng/ g后 ,胎脑、胎肝及胎心之 1 2 5I- b FGF分别为 (3.5± 2 .1)、(7.3± 4 .9)、(12 .1± 4 .6 ) ng/ g,差异有显著性 (t>2 .5 8,P<0 .0 1) ;各组增殖神经元计数分别为 (4 .5± 2 .4 )、(5 .8± 3.1)、(17.2± 5 .4 )、(18.1± 5 .8)个 / HP,差异有非常显著性 (F =12 8,P<0 .0 0 1) ;各组存活神经元总数分别为 (6 5± 9)、(30± 8)、(5 6± 8)、(5 9± 7)个 / HP,差异有非常显著性 (F=12 8,P<0 .0 0 1)。 结论 b FGF能够通过胎盘屏障并促进宫内窘迫胎鼠神经元增殖并增加神经元存活数。
Objective To investigate if basic fibroblast Growth factor (bFGF) can penetrate placental barrier, alleviate rat brain damage and stimulate fetal rat neurons proliferation. Methods BFGF labeled with 125 I was injected peritoneally to pregnant rats, and tissue distribution of radioactivity of 125 I in different organs was detected. Thirty two of pregnant Waister rats were randomly divided at 15 d of gestational age into four groups: normal control, uteri distressed control, bFGF treatment, and bFGF prevention. Fetal rats in the latter three groups suffered from distress in uteri in an animal model of perinatal asphyxia. Proliferated neurons in fetal rat brains were counted in each group. Results 125 I bFGF has been found in fetal brain, heart, lung and spleen, etc. Under high power microscope, proliferated neurons in fetal rat brains were counted in each group, they are: (4.5 ±2.4), (5.8±3.1), (17.2±5.4); (18.1±5.8), ( F=128,P <0.001); all alive neurons in fetal rat Brains are: (65±9),(30±8), (56±8), ( 59±7),( F=105,P <0.001). Conclusions BFGF can penetrate placental barrier, and protect brain cells from hypoxia and stimulate neurons proliferation.
出处
《中华围产医学杂志》
CAS
2003年第2期93-95,共3页
Chinese Journal of Perinatal Medicine
关键词
成纤维细胞生长因子2
胎儿窘迫
脑损伤
Fibroblast growth factor 2
Rats, wistar
Placenta
Fetal distress
Neurons
