摘要
目的 碱性成纤维细胞生长因子 (b FGF)能否通过血脑屏障 (BBB)发挥其神经营养因子作用尚不明确。方法 本文采用 1 2 5 I-标记 b FGF经腹腔注射后 2 5分钟检测全身各组织放射活度 ,计算 b FGF的组织含量。结果 表明脑 b FGF含量成年鼠为 17.36± 8.30 pg/ mg,新生鼠为 31.96± 10 .11pg/ mg新生鼠缺氧组为 5 6 .6 8± 15 .79pg/ mg,各组相差具有显著意义 (P<0 .0 5～ 0 .0 1) ;以同一时相血液 b FGF含量为参照 ,成年鼠脑 b FGF含量相当于全血含量的6 .5 1± 3.80 % ,新生鼠为 14.0 9± 3.12 % ,新生鼠缺氧组为 2 6 .2 4=11.83% ,各组相差具有显著意义 (P〈0 .0 5～ 0 .0 0 5 )。结果提示 ,b FGF可通过血脑屏障。对新生鼠及其缺氧状态下应用b FGF,脑内 b FGF含量较成年鼠显著增多 。
It is still debated if basic fibroblast growth factoor (bFGF) could penetrate blood brain barrier (BBB) to act as a neurotrophic factor. To make it clear we labeled bFGF with 125 I and then tested the radioactivity of 125 I and counted the contents of bFGF in various organs after injection of 125 I bFGF into abdiminal cavity. The results showed that the bFGF xontents of brain were respectively 17.36±8.30pg/mg in adult rats,31.96±10.11 pg/mg in newborn rats and 56.681±15.79pg/mg in newborn rats with hypoxidosis. There were a significant defference among these three groups (P<0.05～0.005). With reference to bFGF contents of blood at same phasic time,percentage of bFGF contents in brain to blood were respectively 6.51±3.80% in adult rats, 14.09±3.12% in newborn rats and 26.24±11.85% in newborn rats with hypoxidosis. A significant defference was found among the three groups (p<0.05～0。005). The present result indicates that bFGF could penetrate BBB. By means of periferal injection of bFGF, the bFGF. contents of brain in newborn rats and those with hypoxidosis increase more greeatly than that in adult rats. Therefor, exogenous bFGF will have more curative effect on central nervous system n newbron rats and those with hypoxidosis.
出处
《新生儿科杂志》
1999年第3期116-118,共3页
The Journal of Neonatology
