摘要
借助麦蚜人工饲料研究明确了小麦几种主要次生物质单宁酸、总酚 (没食子酸 )和香豆素对麦长管蚜Sitobionavenae的存活、生长和发育有明显的抑制作用 ,其抗蚜阈值浓度分别为 0 0 6 %、 0 0 8%和 0 0 6 5 %。用昆虫酶系体外抑制法 ,研究上述三种次生物质的抗蚜阈值浓度对麦长管蚜的糖转化酶和解毒酶活力的影响 ,结果表明 :0 0 6 %单宁酸强烈抑制麦长管蚜蔗糖酶、海藻糖酶、羧酸酯酶和谷胱甘肽S 转移酶的活力 ;0 0 8%没食子酸显著抑制蔗糖酶、羧酸酯酶和谷胱甘肽S 转移酶的活力 ;0 0 6 5 %香豆素极显著地抑制谷胱甘肽S 转移酶活力。
The anti-herbivore effects of major secondary substances of wheat (Triticum aestivum L.), including tannic acid, total phenolic compound and coumarin, on the grain aphid, Sitobion avenae, were investigated by adding them to a chemically defined diet. The impact of these secondary chemicals on the activity of amylase, invertase, trehalase, esterase (CarE) and glutathione S-transferase (GST) in grain aphids was measured in vitro. The results show that tannic acid, total phenolic compound and coumarin significantly decreased nymphal survival, development and weight gain, with their resistant threshold concentrations being 0.06%, 0.08% and 0.065%, respectively. At these concentrations, tannic acid strongly inhibited the activity of invertase, trehalase, CarE and GST and gallic acid (total phenolic chemicals) greatly decreased the activity of invertase, CarE and GST. However, coumarin only strongly inhibited GST activity.
出处
《昆虫学报》
CAS
CSCD
北大核心
2003年第2期144-149,共6页
Acta Entomologica Sinica
基金
国家重点基础研究发展规划"973"项目 (G2 0 0 0 0 16 2 1110 )
关键词
麦长管蚜
糖转化酶
解毒酶
次生物质
抗蚜性
Sitobion avenae
sugar invert enzymes
detoxification enzymes
secondary substances
anti-herbivore effect
