摘要
根据已公布的金黄色葡萄球菌耐热核酸酶 nuc基因的序列 ,设计并合成一对特异性的引物 ,以鼠金黄色葡萄球菌临床分离株 SA.m0 1 0 9的基因组 DNA为模板 ,利用 PCR技术对其耐热核酸酶 nuc基因片段进行扩增 ,获得了预计大小的片段。将这一片段克隆到质粒载体 p GEM- T中 ,对重组质粒 p GEM- NUC进行限制性内切酶分析、PCR鉴定和克隆片段的序列测定 ,证实了克隆片段的可靠性。
According to the sequence of the nuc gene, which encodes the thermostable nuclease (TNase) of Staphylococcus aureus (S.aureus), a pair of oligonucleotide primers which are special to the sequence of nuc gene were designed and synthesized. Using the genome DNA of the clinically isolated strain of S.aureus SA.m0109 as template, the fragment flanking nuc gene was amplified by Polymerase Chain Reaction (PCR). The expected size fragment was obtained and was cloned into the plasmid pGEM-T. The recombinant plasmid of pGEM-NUC was identified by restriction enzyme analysis, PCR and sequencing, which proved completely its validity.
出处
《上海实验动物科学》
2003年第1期3-6,共4页
Shanghai Laboratory Animal Science
基金
"十五"国家科技攻关计划"国家遗传工程小鼠资源库的建立"(项目编号 :2 0 0 1BA70 113)
