摘要
通过PCR方法扩增噬菌体ΦX174的裂解基因E,将其与温控表达载体pBV220连接,成功构建了温控裂解表达载体;将该载体转入猪致病性大肠杆菌O139菌株中,温控诱导裂解蛋白的表达,制备O139的菌影,并对其裂解效率做了初步研究。结果显示,成功克隆了裂解基因E,构建了温控裂解表达载体pBV-E,pBV-E在猪大肠杆菌O139中通过温控表达裂解蛋白能够抑制细菌的增殖,并使活菌数下降3个指数。结果表明,制备的猪大肠杆菌O139菌影为猪大肠杆菌O139新型菌影疫苗的研制奠定了基础。
Lysis gene E of phage φ174 was amplified by PCR. Then the amplified fragment was cloned into thermostat expression vector pBV220 to construct a thermostat lysis expression vector pBV-E. The pBV-E was transformed into pig pathogenic Escherichia coli 0139, and the E. coli O139 ghost was prepared by controlled expression of the lysis gene E. The result showed that the lysis gene E was successfully amplified and the thermostat lysis expression vector pBV-E was successfully constructed,and pBV-E could inhibit bacterium replication by thermostat expression of the lysis protein in E. coli O139, and the ghost could cause a reduce of the index of viable bacterium count to three. In conclusion,the prepared E. coli O139 ghost provided the foundations for studying a novel vaccine of ghost.
出处
《中国兽医科学》
CAS
CSCD
北大核心
2013年第5期476-479,共4页
Chinese Veterinary Science
基金
滨州市科技发展计划项目(2011ZC0707)
关键词
裂解基因E
温控表达载体
裂解效率
lysis gene E
thermostat expression vector
lysis efficiency
