摘要
目的 全反式维甲酸(ATRA)对肿瘤细胞具有广泛的抑制增殖、促进分化作用。本研究通过体外培养检测ATRA对K562细胞是否具有诱导分化作用。方法 采用联苯胺染色、瑞氏染色、非特异性酯酶染色、硝基四唑氮蓝还原试验4种不同的染色方法及流式细胞术,观察经1μmol/L及2.5μmol/L ATRA诱导1d,4d,5d后,K562细胞出现诱导分化特征。结果 1μmol/L及2.5μmol/L ATRA均可诱导K562细胞向粒系分化。培养4d,1μmol/L ATRA组61.5％的K562细胞、2.5μmol/L组39％的K562细胞显示出向粒系成熟方向分化;培养5d,处理组的分化细胞数仍明显高于对照组(P<0.05),但两种浓度ATRA的诱导分化强度无统计学差异(P>0.05)。且均未出现向红系或单核细胞方向分化的特征。流式细胞仪检测ATRA诱导前后CD13及CD71的表达,1μmol/L ATRA诱导K562细胞1d,CD13抗原表达阳性率为8.0％,2.5μmol/L组则为6.7％,均高于对照组(2.1％)。培养5d,1μmol/L和2.5μmol/L AFRA组的CD13分别升高到28.1％,37.8％,而CD71则分别下降至1.2％和0.9％。与对照组比差异均具有显著性(P<0.05)。结论 ATRA可诱导K562细胞向粒系成熟方向分化。
Objective ATRA can restrain proliferation and promote differentiation in various tumor cells. The aim of the study is to investigate the differentiation characteristics of K562 induced by ATRA. Methods Morphology (Benzidine staining, Wright's staining, NSE staining and NBT recovery test) and flow cytometry were used to observe the differentiation characteristics of K562 after co-incubation with 1 μmol/L and 2.5 pmol/L ATRA for 1 d, 4 ds, and 5 ds. Results Co-incubated for 4 ds, 61.5% K562 cells in the 1 μmol/L ATRA group and 39% K562 cells in the 2.5 μmol/L ATRA group showed some evidence of myeloid maturation, but no evidence of erythroid or monocytoid maturation. Cb-incubated for 5 ds, the percentage of differentiated K562 cells was much higher than that in the control group. One day after induction by 1 μmol/L ATRA or 2.5 μmol/L ATRA, the expression of CD13 was 8.0% and 6.7%, respectively, which was higher than that in the control group (2.1%). Five days after induction by 1 μmol/L ATRA or 2.5 μmol/L ATRA, the expression of CD13 increased to 28.1 % and 37.8 % , respectively, while the expression of CD71 decreased to 1.2 % and 0.9 % respectively. The differences between the ATRA groups and the control group were significant ( P < 0.05). CD71 decreased from 9.7% and 10.8% in the 1 μmol/L and 2.5 μmol/L ATRA groups on day 1 to 1.2% and 0.9% on day 5, while the CD13 expression level increased from 8.0% and 6.7% to 28.1% and 37.8% , respectively. Conclusions ATRA can induce K562 to differentiate into myeloid linage. [Chin J Contemp Pediatr, 2003, 5(1): 8-11]
出处
《中国当代儿科杂志》
CAS
CSCD
2003年第1期8-11,共4页
Chinese Journal of Contemporary Pediatrics
