摘要
目的 检测诱生型一氧化氮合酶 (iNOS)在老年豚鼠耳蜗的表达。 方法 将 2 0只豚鼠分 2组 :实验组豚鼠年龄为 3 3～ 3 5个月之间 ,对照组豚鼠年龄为 2～ 3个月。测定两组豚鼠听觉脑干诱发电位 (ABR)听阈 ,用免疫组织化学方法检测iNOS在两组豚鼠耳蜗的表达。 结果 对照组和实验组豚鼠ABR平均听阈分别为 (2 2 0± 5 3 )dBspL、(5 0 0± 10 5 )dBspL ,且两组间差异有显著性 (P <0 0 5 )。iNOS在实验组耳蜗的表达呈阳性 ,阳性区域主要在血管纹和螺旋神经节细胞。iNOS在对照组耳蜗的表达呈阴性。 结论 iNOS在老年豚鼠耳蜗中呈阳性表达 ,提示一氧化氮 (NO)
Objective To determine the expression of inducible nitric oxide synthase (iNOS) in cochlea of the aged guinea pigs. Methods Twenty guinea pigs were divided into two groups: experimental group(33 35 months old) and control group(2 3 months old). The threshold of auditory brain stem response (ABR) of two groups was measured, iNOS was examined in the cochlea of both groups using immunohistochemical method. Results The ABR threshold of coantor group was (22 0±5 3) dBspL, and the experimental group was (50 0±10 5) dBspL, There was a significant difference in ABR threshold between two groups ( P <0 05).The iNOS was expressed in stria vascularis and spiral ganglion cell of the cochlea of experimental group, and was not expressed in the control group. Conclusions The expression of iNOS was positive in the cochlea of the aged guinea pigs. It suggests that nitric oxide (NO) takes a very important role in the aging process of cochleas of guinea pigs.
出处
《中华老年医学杂志》
CAS
CSCD
北大核心
2003年第3期162-164,共3页
Chinese Journal of Geriatrics
关键词
耳蜗
一氧化氮合酶
免疫组织化学
Cochlea
Nitric oxide synthase
Immunohistochemistry
