摘要
克隆LRP16基因启动子分子,并对启动子特征进行分析,预测启动子区调控元件,为深入研究LRP16基因的表达调控机制奠定基础。在NCBI的人类基因组数据库中截取并下载LRP16基因转录起始位点5'侧翼区2.7kb的基因组序列,设计PCR引物,从健康外周血单个核细胞中扩增;利用Genomatix程序对5'侧翼区近1000bp进行启动子特征分析。获得了与GenBank序列一致、长度为2.7kb的LRP16基因启动子DNA序列,该序列具有典型的真核生物RNA聚合酶II启动子特征及多个核受体结合位点,如α视黄酸受体及RAR相关孤生受体。
To explore the possible regulation mechanism of LRP16gene e xpression and to clone LRP16gene pro-moter molecule.A2.7kb DNA sequence o f LRP165'-end was obtained from NCBI by BLAST software.The2.7kb long ta rget sequence from a healthy blood donor DNA sample were amplified by PCR amplif ication,then the prod-uct were identified by DNA sequencing and nest PCR.The verified sequence was analyzed by Genomatix on line.The2.7kb long target seq uence was the same as the GenBank described and the sequence has the typical eu cary-ocytic promoter chacracter with a few nuclear receptor binding motif,such as RAR-alpha and ROR.A known gene promoter sequence can be freely obtained fr om NCBI database,and this is very useful for the gene promoter cloning,promo ter analysis and gene function and classification prediction.
出处
《生物技术通讯》
CAS
2003年第1期20-22,共3页
Letters in Biotechnology
