摘要
发现新的白血病相关基因及探讨白血病的发生机制。方法 :应用分子克隆技术RLGS及RACE技术自急性髓细胞白血病骨髓标本中克隆新的白血病相关基因。结果 :克隆到一个长度为 2 948bp的DNA片段 ,经国际基因库同源检索证明为一未知基因的一部分 ,并定位于第 11号染色体长臂 11区 ,进一步克隆到这一基因的全长cDNA ,其长度为 980bp ,并被国际基因库以新的人类基因LRP16收录入库。结论 :RLGS及RACE技术对于发现白血病相关基因是一种有效的方法 ,LRP16基因的发现对于探讨白血病发生机制具有一定意义。
Objective:The purpose of this paper is to clone novel leukemia associated gene in acute myeloid leukemia (AML).Methods:The Restriction landmark genomic scanning (RLGS),Southern blot, Northern blot and rapid amplification of cDNA end (RACE) technique were performed on genomic DNA or RNA from AML and normal bone marrow (BM) samples.Results:An abnormal 2948bp fragment due to a distinct DNA methylation on NotI site in relapse sample from AML was cloned. The Sequence represents part of a new gene LRP16 which is located on chromosome 11q11. The full length cDNA sequence 980bp was cloned by RACE technique. Conclusion:The results indicate that RLGS and RACE technique are effective methods to find leukemia associated gene in AML. The finding of LRP16 gene is important to investigate mechanism of AML.
出处
《军医进修学院学报》
CAS
2000年第2期81-84,共4页
Academic Journal of Pla Postgraduate Medical School
基金
国家自然科学基金资助项目! (39970 82 )
"九五"军队科研基金项目 !(98M14 1)
