摘要
利用显微注射的方法,分别将三株不同类型的经过遗传修饰的中靶ES细胞注射到C57BL/6J小鼠的囊胚中,通过胚胎移植将注射后的囊胚引入受体小鼠子宫中,分别获得了不同整合度的嵌合体小鼠,将高嵌合度小鼠与C57BL/6J小鼠杂交,对这些仔鼠进行PCR及Southern鉴定的结果表明,三株修饰后的ES细胞均能整合入生殖系,得到了棕褐色子代鼠,表明获得了种系嵌合体小鼠。
Three ES cell lines modified by different genetic manipulation were introduced into blastocysts of C57BL/6J mice by microinjection respectively.The injected blastocysts were transplanted into the uterus of pseudopregnant mice.Chimeras with different degrees of the ES cell integration were obtained.Chimeras were crossed with C57BL/6J mice and the offspring carring the modified alleles were identified by PCR and Southern blot hybridization.These results demonstrated that the germ-line transmission mice from three different genetic modified ES cell lines were obtained.
出处
《生物技术通讯》
CAS
2003年第1期1-3,共3页
Letters in Biotechnology
基金
国家高技术研究发展计划项目(2001AA216081)
国家自然科学基金项目(30070837和30025028)
关键词
遗传修饰
小鼠
胚胎干细胞
种系嵌合体
显微注射
microinjection
genetic modification
embryonic stem cell
germ-line chimera
