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Quick search of differentially expressed genes of EB virus resulting immortal human B cells by suppression subtractive hybridization 被引量：5

Quick search of differentially expressed genes of EB virus resulting immortal human B cells by suppression subtractive hybridization
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摘要 AIM:To clone and identify genes differentially expressed in the EB virus transformed human B cell.METHODS:Suppression subtractive hybridization was used to construct the library which contains the differentiately expressed cDNAs in EB virus transformed human B cell.Then the isolated genes were cloned and sequenced and identifed by RT PCR.Nucleic acid homology searches were performed using the BLAST program.RESULTS:By this technique,4 differentiately expressed gene cDNA fragments of EB virus transformed human B cell were obtained.CONCLUSION:SSH is an effective method to isolate differentiately expressed genes. AIM:To clone and identify genes differentially expressed in the EB virus transformed human B cell.METHODS:Suppression subtractive hybridization was used to construct the library which contains the differentiately expressed cDNAs in EB virus transformed human B cell.Then the isolated genes were cloned and sequenced and identifed by RT PCR.Nucleic acid homology searches were performed using the BLAST program.RESULTS:By this technique,4 differentiately expressed gene cDNA fragments of EB virus transformed human B cell were obtained.CONCLUSION:SSH is an effective method to isolate differentiately expressed genes.
作者 李茜 朱振宇 梁雪清 李满枝 黄必军
机构地区 DepartmentofBiochemistrySunYat-senMedicalCollege
出处 《中国临床康复》 CSCD 2003年第5期872-873,T002,共3页 Chinese Journal of Clinical Rehabilitation
关键词 抑制性消减杂交技术 克隆 EB病毒 人B淋巴细胞永生化 差异性表达基因 herpesvirus 4,human genetic techniques B lymphocytes
分类号 R373 [医药卫生—病原生物学] Q78 [生物学—分子生物学]
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中国临床康复
2003年 第5期

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