摘要
应用 Ames 试验对亚硒酸钠(Na_2SeO_3)、茶多酚(GTP)、维生素E(VE)、L—色氨酸和叶酸等五种化合物进行黄曲霉毒素B_1(AFB_1)诱导鼠伤寒沙门氏菌TA98、TA100突变株回复突变作用实验.当五种化合物加入培养基时,有Na_2SeO_3、GPT和VE等三种化合物对AFB_1诱导TA98、TA100引起的回变菌落数明显减少.结果表明这三种化合物对AFB_1的诱变效应有明显的拮抗作用,且显示良好的剂量效应关系.提示对预防肝癌可能有意义.
Ames test was used to test Na_2Seo_3,GPT.VE for their anti-mutagenic effect on AFB_1 induced mutagenic activity in TA98 and TA100 mutants.Aflatoxin B_1was used as the mutagen.The re- vertants induced by AFB_1 were significantly decreased when these compounds were added into the medi- um.The results revealed that 3 Na_2Seo_3,GPT and VE of the 5 compounds showed remarkable inhibition effect on AFB_1 induced mutagenic activity for strains TA98 and TA100.the fact that thses 3 compounds possess remarkable anti-mutagenic effect indicate that they may be of practical value in the field of chemoprophylaxis of liver cancer in men.
关键词
黄曲霉毒素
致癌因素
肝肿瘤
compouns
aflatoxins
antimutagenicity test
mutagenicity
chemoprevention of cancer
