摘要
目的 研究健脾降脂颗粒中绿原酸的含量测定方法。方法 采用高效液相法。色谱柱YWGC18(2 5 0mm× 4 6mm ,10 μm) ,室温 ,10 %乙腈─冰醋酸 (5∶0 1)为流动相 ,流速为 1mL/min ,检测波长 32 9nm。结果 绿原酸在 0 0 2 0 8～ 0 10 4 μg范围内呈线性关系 (r =0 9994 ,n =5 ) ,平均加样回收率为 96 2 3% ,RSD为 0 798%。结论 本方法准确、可行 。
Objective To establish a method used for quantitatively determining chlorogenic acid in Jianpijiangzhi Granules. Method HPLC techniques were adopted: the YWGC 18 column (250 mm×4 6 mm, 10 μm) was used, the experiment was carried out at room temperature, the mobile phase was 10% acetonitrile glacial acetic acid (5∶0 1) at a flow rate of 1 ml/minute, and the detecting wavelength was 329 nm. Results The linear range of chlorogenic acid was between 0 0208 μg～0 104 μg ( r=0.9994, n=5 ), the average sample recovery was 96 23%; and RSD was 0 798%. Conclusion This method is accurate and practical, and can be used for controlling the quality of Jianpijiangzhi Granules.
出处
《北京中医药大学学报》
CAS
CSCD
北大核心
2003年第2期50-52,共3页
Journal of Beijing University of Traditional Chinese Medicine
