摘要
[目的]观察通痹灵对胶原诱导性关节炎(CIA)大鼠关节滑膜组织炎症和血管内皮生长因子(VEGF)表达水平的影响,以探讨通痹灵治疗类风湿性关节炎(RA)的作用机理。[方法]大鼠尾根部皮下注射Ⅱ型胶原蛋白建立CIA模型,分组给药治疗.给药37d后处死动物,对动物膝关节滑膜组织病理切片后分别常规HE染色和免疫组化染色。对HE染色切片用显微镜观察炎症细胞浸润、巨噬细胞增生和纤维增生情况,对免疫组化染色切片观察血管内皮生长因子(VEGF)阳性细胞情况以了解各组动物关节滑膜VEGF的表达水平。[结果]HE染色切片显示通痹灵高剂量组滑膜炎症细胞浸润、巨噬样A型细胞增生和纤维组织增生的程度均比模型组低(P<0.05或P<0.01),并与正常组相仿(P>0.05)。免疫组化染色切片显示通痹灵和雷公藤高剂量组均能降低滑膜VEGF表达水平(P<0.05)。[结论]通痹灵有改善大鼠关节滑膜炎症,抑制滑膜VEGF表达作用,这可能是通痹灵治疗关节炎取得临床疗效的机理之一。
[ObjectivejTo explore the therapeutic mechanism of Tongbiling (TBL) prescription for rheumatoid arthritis (RA) by observing the effects of TBL on inflammation and expression of vascular endothelial growth factor (VEGF) in synovium of rats with collagen-induced arthritis (CIA) .[Methods]CIA rat models were established by subcutaneous injection of type II collagen. Pathological sections of synovium in knee joint were made after medication for 37 days and then stained by HE method and immunohistochemical method respectively. Infiltration of inflammatory cells and hyperplasia of macrophages A and fibrous tissue in HE-stained pathological sections were observed under microscope and expression of VEGF was observed by semi-quantitative immunohistochemical method. [ Results ] Infiltration of inflammatory cells and hyperplasia of macrophages A and fibrous tissue in high-dose TBL group were inferior to those in model group (P < 0.05 or P < 0.01) and similar to those in normal group (P > 0.05); both TBL and Radix Tripterygii Wilfordii at high dose decreased the expression of VEGF (P <0.05 as compared with the model group) . [Conclusion]The improvement of synovial inflammation and the decrease of VEGF may be the therapeutic mechanisms of TBL for RA and the increase of neoformative vessels may be the important pathogenesis of RA.
出处
《广州中医药大学学报》
CAS
2003年第1期4-7,共4页
Journal of Guangzhou University of Traditional Chinese Medicine
基金
国家自然科学基金重点项目资助(编号:39930230)
广东省自然科学基金重点项目资助(编号:020656)
关键词
类风湿关节炎
中药疗法
通痹灵
药理学
桂枝芍药知母汤
滑膜
药物作用
血管生成因子
ARTHRITIS, RHEUMATOID/TCD therapy
TONGBILING/phamiacology
GUEHI SHAOYAO ZfflMU DECOCnON/phaimacology
SYNOVIAL MEMBRANE/drug effects
ENDOTHELIALS, VASCULAR/drug effects
VASCULAR ENDOTHEIIAL GROWTH FACTOR
