摘要
为建立保健食品中洛伐他丁的高压液相色谱测定方法 ,首先明确了洛伐他丁酸式、内酯和甲酯 3种结构中内酯为测定结构 ,通过研究提取、净化方法 ,尤其是温度、pH值等重要影响因素和仪器分析条件后 ,确定了可行的检测方法。能在 2d内以一种结构进行洛伐他丁测定 ,保证结果能准确反映样品的实际情况。本方法低加标浓度平均回收率为 96 3% ,高加标浓度平均回收率为10 7 4 % ,相对标准偏差 3 0 % ,最小检出限 0 0 8mg ,结果符合食品理化分析的要求 ,适用于原料、胶囊、保健醋等试样的检测。 6年来的稳定性实验表明 。
For developing the determination method of lovastatin in health foods by HPLC, we first inspected the molecular structure of lovastatin and ascertained that lactone is the best one for determination among the acid, lactone and methyl ester forms. The feasible method was decided through a complete research of extraction and purification method, important influence factors especially temperature, pH and HPLC operation conditions. Lovastatin in one structure was determined within 2 days, which accurately mirrors the real state of the sample. Recoveries of higher and lower standard spiked were 107 4% and 96 3% resspectively, the RSD was 3 0%. The results fitted the demand of food analysis. This method can be used in the analysis of raw materials, capsules and functional vinegars. Results of 80 stability experiments showed that solid state samples were more stable.
出处
《中国食品卫生杂志》
2003年第2期125-128,共4页
Chinese Journal of Food Hygiene
基金
卫生部保健食品专项基金课题 (BJ-0 1-2 0 )~~
关键词
保健食品
洛伐他丁
测定方法
高压液相色谱法
Dietary Supplements
Food Analysis
Lovastatin
Chromatography, High Pressure Liquid
