摘要
目的:本文建立高效液相色谱法测定保健食品中(以纳豆红曲茶多酚软胶囊为例)洛伐他汀的方法。方法:样品经乙腈提取后,用液相色谱C18柱分离,二元流动相洗脱,238 nm波长下紫外检测器检测,测定精密度、准确度。结果:洛伐他汀在10~200μg·mL^(-1)线性关系良好,相关系数>0.9999,相对标准偏差为0.62%。当加标浓度为0.5 mg时,回收率为98.8%;当加标浓度为1.0 mg时,回收率为102.7%;当加标浓度为1.5 mg时,回收率为99.7%。结论:本方法检测灵敏度高、操作简单、定性定量准确、针对性强,为纳豆红曲茶多酚软胶囊质量控制和深度开发提供了科学依据。
Objective:To establish a method for the determination of lovastatin in health food by high performance liquid chromatography.Method:After being extracted with acetonitrile,the sample was separated using a liquid chromatography C18 column,eluted with a binary mobile phase,and detected with a UV detector at a wavelength of 238 nm for precision and accuracy.Result:The linear range of lovastatin was 10~200μg·mL^(-1),the correlation coefficient was more than 0.9999,and the relative standard deviation was 0.62%.When the concentration was 0.5 mg,the recovery rate was 98.8%.When the concentration was 1.0 mg,the recovery rate was 102.7%.When the concentration was 1.5 mg,the recovery rate was 99.7%.Conclusion:This method has high sensitivity,simple operation,accurate qualitative and quantitative,and strong pertinence.It provides a scientific basis for the quality control and in-depth development of natto monascus tea polyphenol soft capsule.
作者
季加才
孟凡荣
JI Jiacai;MENG Fanrong(Shandong Runda Testing Technology Co.,Ltd.,Weifang 261000,China)
出处
《食品安全导刊》
2023年第8期81-84,共4页
China Food Safety Magazine
关键词
高效液相色谱法
洛伐他汀
纳豆红曲茶多酚软胶囊
high performance liquid chromatography
lovastatin
natto monascus tea polyphenol soft capsules
