摘要
在海藻糖生产菌的筛选过程中 ,微生物胞内酶转化淀粉生成的产物复杂 ,将产物逐一纯化是非常烦琐的 ,但又必须确证产物中是否含有海藻糖。本文将薄层层析、高效液相电喷雾电离质谱联用及核磁共振等分析手段综合应用于海藻糖生产菌株的筛选 ,在酶反应产物不必被纯化的前提下 ,准确、快捷地鉴定了酶反应产物中的未知糖组分 ,最终证明食尼古丁节杆菌 (Arthrobacternicotinovorus)D 97利用淀粉或麦芽寡糖的酶反应产物中含有海藻糖。该方法在筛选海藻糖及其它功能性葡二糖生产菌株时较为严密。
In the isolating process of stains whose endocellular enzymes can produce trehalose on starch or maltooligosaccharides, we discovered the components of enzymatic reactant were complicated and it was difficult to purify them each other, however, we have to know quickly whether there was tehalose in the enzymatic reactant above. In order to make it clearly, thinner layer chromatography, high performance liquid chromatography-electrospray ionization mass spectrometry and 13C-nuclear magnetic resonance were adopted. The unknown oligosaccharide produced by Arthrobacter nicotinovorus D-97 was identified rapidly and this unknown oligosaccharide needn't be purified from the enzymatic reactant solely. The production on starch or maltooligosaccharides by endocellular enzymes of Arthrobacter nicotinovorus D-97 is trehalose. This research method in our paper can be applied in the isolating process of production trehalose and other functional glucobioses strains.
出处
《微生物学报》
CAS
CSCD
北大核心
2003年第1期104-110,共7页
Acta Microbiologica Sinica
关键词
海藻糖生产菌株
筛选过程
产物鉴定
薄层层析
高效液-电喷雾电离质谱联用
核磁共振
trehalose, thinner layer chromatography, high performance liquid chromatography-electrospray ionization mass spectrometry, 13C-nuclear magnetic resonance
